Cloned (Comment) | Organism |
---|---|
GEDH1, phylogenetic tree and analysis, sequence comparison, expression in Escherichia coli | Ocimum basilicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 66% inhibition at 5 mM | Ocimum basilicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
geraniol | pH 9.5 | Ocimum basilicum | |
0.037 | - |
nerol | pH 9.5 | Ocimum basilicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by Na+, K+, NH4+, Ca2+, and Mg2+ | Ocimum basilicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + NADP+ | Ocimum basilicum | the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo, the geraniol,/nerol pathway involves sveral dehydrogenases, overview | geranial + NADPH | - |
ir | |
nerol + NADP+ | Ocimum basilicum | - |
neral + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ocimum basilicum | Q2KNL6 | GEDH1; cv. Sweet Dani, gene GEDH1 | - |
Purification (Comment) | Organism |
---|---|
recombinant GEDH1 from Escherichia coli by anion exchange chromatography | Ocimum basilicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | highest activity in young leaves decreasing to 60% in older leaves | Ocimum basilicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-buten-1-ol + NADP+ | 22.7% of the activity with geraniol | Ocimum basilicum | 3-methyl-2-butenal + NADPH | - |
? | |
3-methyl-3-buten-1-ol + NADP+ | 6.5% of the activity with geraniol | Ocimum basilicum | 3-methyl-3-butenal + NADPH | - |
? | |
benzylalcohol + NADP+ | 5.5% of the activity with geraniol | Ocimum basilicum | benzaldehyde + NADPH | - |
? | |
cinnamyl alcohol + NADP+ | 53.0% of the activity with geraniol | Ocimum basilicum | cinnamyl aldehyde + NADPH | - |
? | |
cis-3-hexenol + NADP+ | 13.3% of the activity with geraniol | Ocimum basilicum | cis-3-hexenal + NADPH | - |
? | |
citronellol + NADP+ | 16.6% of the activity with geraniol | Ocimum basilicum | citronellal + NADPH | - |
r | |
geraniol + NADP+ | the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo, the geraniol,/nerol pathway involves sveral dehydrogenases, overview | Ocimum basilicum | geranial + NADPH | - |
ir | |
geraniol + NADP+ | the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo | Ocimum basilicum | geranial + NADPH | - |
? | |
hexanol + NADP+ | 31.6% of the activity with geraniol | Ocimum basilicum | hexanal + NADPH | - |
? | |
additional information | no activity with coniferyl alcohol, menthol, carveol, dihydrocarveol, 3,7-dimethyl-octanol, ethanol, butanol, and isobutanol | Ocimum basilicum | ? | - |
? | |
nerol + NADP+ | - |
Ocimum basilicum | neral + NADPH + H+ | - |
r | |
nerol + NADP+ | 101% of the activity with geraniol | Ocimum basilicum | neral + NADPH + H+ | - |
r | |
phenylethanol + NADP+ | 10.2% of the activity with geraniol | Ocimum basilicum | phenylethanal + NADPH | - |
? | |
phenylpropanol + NADP+ | 22.1% of the activity with geraniol | Ocimum basilicum | phenylpropanal + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GEDH1 | - |
Ocimum basilicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Ocimum basilicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10 | over 72% of maximal activity at pH 8.5 and pH 10.0 | Ocimum basilicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Ocimum basilicum | |
NADPH | - |
Ocimum basilicum |