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Literature summary for 1.1.1.183 extracted from

  • Iijima, Y.; Wang, G.; Fridman, E.; Pichersky, E.
    Analysis of the enzymatic formation of citral in the glands of sweet basil (2006), Arch. Biochem. Biophys., 448, 141-149.
    View publication on PubMed
Search for more literature for 1.1.1.183

Cloned(Commentary)

Cloned (Comment) Organism
GEDH1, phylogenetic tree and analysis, sequence comparison, expression in Escherichia coli Ocimum basilicum

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ 66% inhibition at 5 mM Ocimum basilicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 geraniol pH 9.5 Ocimum basilicum
0.037
-
 nerol pH 9.5 Ocimum basilicum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not affected by Na+, K+, NH4+, Ca2+, and Mg2+ Ocimum basilicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geraniol + NADP+ Ocimum basilicum the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo, the geraniol,/nerol pathway involves sveral dehydrogenases, overview geranial + NADPH
-
 ir
nerol + NADP+ Ocimum basilicum
-
 neral + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Ocimum basilicum Q2KNL6 GEDH1; cv. Sweet Dani, gene GEDH1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GEDH1 from Escherichia coli by anion exchange chromatography Ocimum basilicum

Source Tissue

Source Tissue Comment Organism Textmining
leaf highest activity in young leaves decreasing to 60% in older leaves Ocimum basilicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyl-2-buten-1-ol + NADP+ 22.7% of the activity with geraniol Ocimum basilicum 3-methyl-2-butenal + NADPH
-
 ?
3-methyl-3-buten-1-ol + NADP+ 6.5% of the activity with geraniol Ocimum basilicum 3-methyl-3-butenal + NADPH
-
 ?
benzylalcohol + NADP+ 5.5% of the activity with geraniol Ocimum basilicum benzaldehyde + NADPH
-
 ?
cinnamyl alcohol + NADP+ 53.0% of the activity with geraniol Ocimum basilicum cinnamyl aldehyde + NADPH
-
 ?
cis-3-hexenol + NADP+ 13.3% of the activity with geraniol Ocimum basilicum cis-3-hexenal + NADPH
-
 ?
citronellol + NADP+ 16.6% of the activity with geraniol Ocimum basilicum citronellal + NADPH
-
 r
geraniol + NADP+ the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo, the geraniol,/nerol pathway involves sveral dehydrogenases, overview Ocimum basilicum geranial + NADPH
-
 ir
geraniol + NADP+ the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo Ocimum basilicum geranial + NADPH
-
 ?
hexanol + NADP+ 31.6% of the activity with geraniol Ocimum basilicum hexanal + NADPH
-
 ?
additional information no activity with coniferyl alcohol, menthol, carveol, dihydrocarveol, 3,7-dimethyl-octanol, ethanol, butanol, and isobutanol Ocimum basilicum ?
-
 ?
nerol + NADP+
-
 Ocimum basilicum neral + NADPH + H+
-
 r
nerol + NADP+ 101% of the activity with geraniol Ocimum basilicum neral + NADPH + H+
-
 r
phenylethanol + NADP+ 10.2% of the activity with geraniol Ocimum basilicum phenylethanal + NADPH
-
 ?
phenylpropanol + NADP+ 22.1% of the activity with geraniol Ocimum basilicum phenylpropanal + NADPH
-
 ?

Synonyms

Synonyms Comment Organism
GEDH1
-
 Ocimum basilicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Ocimum basilicum

pH Range

pH Minimum pH Maximum Comment Organism
8.5 10 over 72% of maximal activity at pH 8.5 and pH 10.0 Ocimum basilicum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Ocimum basilicum
NADPH
-
 Ocimum basilicum