Cloned (Comment) | Organism |
---|---|
functional overexpression as His-tagged protein in Escherichia coli BL21(DE3) | Streptomyces coelicolor |
Crystallization (Comment) | Organism |
---|---|
5 mg/ml recombinant enzyme in 25 mM phosphate buffer, pH 8.0, with 10% glycerol, and 5 mM NADP+, hanging drop vapour diffusion method, against well solution containing 4.0-4.4 M sodium formate and 10% v/v glycerol, X-ray diffraction structure determination and analysis at 2.5 A resolution | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
actIII orf5 gene, ketoreductase type II | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged active enzyme from Escherichia coli | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polyketid + NADPH | enzyme acts on the nascent polyketid chain from polyketid synthase reaction and reduces the carbonyl group at C-9 | Streptomyces coelicolor | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | in presence of NADPH, in absence of cofactor the enzyme forms mixtures of dimer, monomer, and tetramer, SDS-PAGE and native PAGE | Streptomyces coelicolor |
Synonyms | Comment | Organism |
---|---|---|
ketoreductase | - |
Streptomyces coelicolor |
KR | - |
Streptomyces coelicolor |
More | enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Streptomyces coelicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Streptomyces coelicolor |