Cloned (Comment) | Organism |
---|---|
from a liver cDNA library, overexpression in Escherichia coli as His-tagged enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-oxonon-2-enal | the substrate is also a mechanism-based inhibitor of the enzyme resulting in 50% inactivation of the enzyme-NADPH complex, presence of cofactor is required for inhibition | Homo sapiens | |
additional information | no inhibition by NADP+ | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
menadione | pH 7.4, 37°C, recombinant enzyme | Homo sapiens | |
0.329 | - |
glutathione-4-oxonon-2-enal | pH 7.4, 37°C, recombinant enzyme | Homo sapiens | |
0.345 | - |
4-oxonon-2-enal | pH 7.4, 37°C, recombinant enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-oxonon-2-enal + NADPH + H+ | molecular modeling of substrate binding in the active site | Homo sapiens | 4-oxononanal + 4-hydroxynon-2-enal + 1-hydroxynon-2-en-4-one + NADP+ | product identification, 4-hydroxynon-2-enal is the major product | ? | |
glutathione-4-oxononanal + NADPH + H+ | conjugate, molecular modeling of substrate binding in the active site | Homo sapiens | glutathione-4-hydroxynonanal + NADP+ | - |
? | |
menadione + NADPH | best substrate | Homo sapiens | ? | - |
? | |
additional information | no activity with 1-hydroxynon-2-en-4-one and 4-hydroxynon-2-enal | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carbonyl reductase | - |
Homo sapiens |
CR | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.6 | - |
glutathione-4-oxonon-2-enal | pH 7.4, 37°C, recombinant enzyme | Homo sapiens | |
220 | - |
4-oxonon-2-enal | pH 7.4, 37°C, recombinant enzyme | Homo sapiens | |
246 | - |
menadione | pH 7.4, 37°C, recombinant enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |