Application | Comment | Organism |
---|---|---|
synthesis | enzyme might be useful for production of ethyl (S)-4-chloro-3-hydroxybutanoate, which is used in the synthesis of pharmacologically and biologically important compounds | Cylindrocarpon sclerotigenum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-dinitrophenol | 19% inhibition at 0.1 mM | Cylindrocarpon sclerotigenum | |
Cd2+ | 16% inhibition at 1 mM | Cylindrocarpon sclerotigenum | |
Cu2+ | 46% inhibition at 1 mM | Cylindrocarpon sclerotigenum | |
dicoumarol | 42% inhibition at 0.3 mM | Cylindrocarpon sclerotigenum | |
DTNB | 56% inhibition at 0.05 mM | Cylindrocarpon sclerotigenum | |
EDTA | 30% inhibition at 1 mM | Cylindrocarpon sclerotigenum | |
Hg2+ | complete inhibition at 1 mM | Cylindrocarpon sclerotigenum | |
additional information | no inhibition by diphenylhydantoin, N-ethylmaleimide, p-chloromercuribenzoate, PMSF, Mg2+, and Zn2+ | Cylindrocarpon sclerotigenum | |
quercetin | 16% inhibition at 0.1 mM | Cylindrocarpon sclerotigenum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24800 | - |
x * 24800, SDS-PAGE | Cylindrocarpon sclerotigenum |
68000 | - |
gel filtration | Cylindrocarpon sclerotigenum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cylindrocarpon sclerotigenum | - |
enzyme CSCR1, strain IFO 31855 | - |
Purification (Comment) | Organism |
---|---|
by sequential column chromatographies involving DEAE ion exchange, phenyl resin, and hydroxylapatite chromatography | Cylindrocarpon sclerotigenum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
125.3 | - |
purified enzyme | Cylindrocarpon sclerotigenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-butanedione + NADPH | - |
Cylindrocarpon sclerotigenum | ? + NADP+ | - |
ir | |
2,3-pentanedione + NADPH + H+ | low activity | Cylindrocarpon sclerotigenum | ? + NADP+ | - |
ir | |
D-xylose + NADPH | very low activity | Cylindrocarpon sclerotigenum | ? + NADP+ | - |
ir | |
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ | highly stereoselective reduction | Cylindrocarpon sclerotigenum | (S)-ethyl 4-chloro-3-hydroxybutanoate + NADP+ | - |
ir | |
ethylpyruvate + NADPH | low activity | Cylindrocarpon sclerotigenum | ? + NADP+ | - |
ir | |
hydroxyacetone + NADPH | low activity | Cylindrocarpon sclerotigenum | ? + NADP+ | - |
ir | |
additional information | enzyme shows highly restricted substrate specificity, overview | Cylindrocarpon sclerotigenum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 24800, SDS-PAGE | Cylindrocarpon sclerotigenum |
Synonyms | Comment | Organism |
---|---|---|
carbonyl reductase | - |
Cylindrocarpon sclerotigenum |
CSCR1 | - |
Cylindrocarpon sclerotigenum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Cylindrocarpon sclerotigenum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Cylindrocarpon sclerotigenum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADH | Cylindrocarpon sclerotigenum | |
NADPH | dependent on, no reverse reaction with NADP+ as cofactor | Cylindrocarpon sclerotigenum |