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Literature summary for 1.1.1.184 extracted from
- Functional significance of a natural allelic variant of human carbonyl reductase 3 (CBR3) (2005), Drug Metab. Dispos., 33, 254-257.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozyme CBR3 V244M, genotyping and polymorphism distribution in 10 different ethnic groups, overview, expression of isozymes CBR V244 and CBR M244 in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| V244M | naturally occuring polymorphism in isozyme CBR3, the isozyme CBR M244 may reflect a faster association-dissociation rate for NADPH/NADP+ per catalytic cycle, residue 244 is not involved in direct cofactor interaction, overview | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0229 | - |
menadione | pH 7.4, 37°C, recombinant isozyme CBR M244 | Homo sapiens |  |
| 0.0246 | - |
menadione | pH 7.4, 37°C, recombinant isozyme CBR V244 | Homo sapiens | |
| 0.085 | - |
NADPH | pH 7.4, 37°C, recombinant isozyme CBR M244 | Homo sapiens | |
| 0.0905 | - |
NADPH | pH 7.4, 37°C, recombinant isozyme CBR V244 | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
isozyme CBR3 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| menadione + NADPH + H+ | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | partial structure modeling | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carbonyl reductase 3 | - |
Homo sapiens |
| CBR3 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.9 | - |
menadione | pH 7.4, 37°C, recombinant isozyme CBR V244 | Homo sapiens | |
| 16.3 | - |
NADPH | pH 7.4, 37°C, recombinant isozyme CBR V244 | Homo sapiens | |
| 20.5 | - |
menadione | pH 7.4, 37°C, recombinant isozyme CBR M244 | Homo sapiens | |
| 26.7 | - |
NADPH | pH 7.4, 37°C, recombinant isozyme CBR M244 | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | binding site modeling | Homo sapiens | |
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Release 2023.1 (January 2023)
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