Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.184 extracted from

  • Zhu, D.; Hua, L.
    Enantioselective enzymatic reductions of sterically bulky aryl alkyl ketones catalyzed by a NADPH-dependent carbonyl reductase (2006), J. Org. Chem., 71, 9484-9486.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of SSCR in Escherichia coli strain BL21(DE3) Sporidiobolus salmonicolor

Organism

Organism UniProt Comment Textmining
Sporidiobolus salmonicolor
-
red yeast, strain AKU 4429
-

Purification (Commentary)

Purification (Comment) Organism
recombinant SSCR from Escherichia coli strain BL21(DE3) Sporidiobolus salmonicolor

Reaction

Reaction Comment Organism Reaction ID
R-CHOH-R' + NADP+ = R-CO-R' + NADPH + H+ substrate specificity, enantioselectivity, and enzyme-substrate docking studies, Ser133 and Tyr177 are important Sporidiobolus salmonicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2-dimethylpropiophenone + NADPH + H+
-
Sporidiobolus salmonicolor 2,2-dimethylpropiophenol + NADP+ over 90% chiral purity of the alcohol product ?
butyrophenone + NADPH + H+
-
Sporidiobolus salmonicolor (1S)-1-phenylbutan-1-ol + NADP+ over 90% chiral purity of the alcohol product ?
ethyl 3,3-dimethyl-2-oxobutyrate + NADPH + H+ stereoselective reaction Sporidiobolus salmonicolor (S)-ethyl 3,3-dimethyl-2-hydroxybutyrate + NADP+ over 90% chiral purity of the alcohol product ?
additional information the enzyme performs enantioselective reductions of sterically bulky aryl alkyl ketones, overview Sporidiobolus salmonicolor ?
-
?
phenyl cyclopropyl ketone + NADPH + H+
-
Sporidiobolus salmonicolor ?
-
?

Synonyms

Synonyms Comment Organism
SSCR
-
Sporidiobolus salmonicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 6.6 assay at Sporidiobolus salmonicolor

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Sporidiobolus salmonicolor