Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sus scrofa |
Crystallization (Comment) | Organism |
---|---|
C-terminal peroxisomal targeting sequence 1 of each subunit is involved in intersubunit interactions and buried in the interior of the tetrameric enzyme | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation of C-terminal peroxisomal targeting sequence SRL to SKL, SHL, SLL, and SL. Mutants SLL and SL are not catalytically active. Mutant SHL is gradually inactivated during incubation at 0°C. Both mutants SHL and SKL are tetramers and show kinetic parameters similar to wild-type | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | C-terminal peroxisomal targeting sequence 1 of each subunit is involved in intersubunit interactions and buried in the interior of the tetrameric enzyme | Sus scrofa | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
4 * 27000, SDS-PAGE and crystallization data | Sus scrofa |
100000 | - |
gel filtration, wild-type and mutants SHL, SKL | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | Q8WNV7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phenylisatin + NADPH | - |
Sus scrofa | ? + NADP+ | - |
? | |
4-benzoylpyridine + NADPH + H+ | - |
Sus scrofa | phenyl-4-pyridylmethanol + NADP+ | - |
? | |
ethyl benzoylformate + NADPH | - |
Sus scrofa | ? + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 27000, SDS-PAGE and crystallization data | Sus scrofa |