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Literature summary for 1.1.1.184 extracted from

  • Ye, Q.; Yan, M.; Yao, Z.; Xu, L.; Cao, H.; Li, Z.; Chen, Y.; Li, S.; Bai, J.; Xiong, J.; Ying, H.; Ouyang, P.
    A new member of the short-chain dehydrogenases/reductases superfamily: purification, characterization and substrate specificity of a recombinant carbonyl reductase from Pichia stipitis (2009), Biores. Technol., 100, 6022-6027.
    View publication on PubMed
Search for more literature for 1.1.1.184

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Scheffersomyces stipitis

General Stability

General Stability Organism
compared to activity in acetate and Tris-HCl, carbonyl reductase shows higher activity using potassium phosphate as buffer at pH 5.5 or pH 7 Scheffersomyces stipitis

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, 20.3% residual activity Scheffersomyces stipitis
sodium dodecylsulfate 0.1% w/v, complete loss of activity Scheffersomyces stipitis
Triton X-100 1% v/v, 88% residual activity Scheffersomyces stipitis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE Scheffersomyces stipitis

Organic Solvent Stability

Organic Solvent Comment Organism
dimethyl sulfoxide 1% v/v, 88.4% residual activity Scheffersomyces stipitis
dimethylformamide 1% v/v, 46% residual activity Scheffersomyces stipitis
tetrahydrofuran 1% v/v, 88.4% residual activity Scheffersomyces stipitis

Organism

Organism UniProt Comment Textmining
Scheffersomyces stipitis A3GF07
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Scheffersomyces stipitis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
337
-
 pH 6.0, 30°C Scheffersomyces stipitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-butanedione + NADPH + H+ 35.2% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis ? + NADP+
-
 ?
2,3-pentanedione + NADPH + H+ 58.6% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis ? + NADP+
-
 ?
3-hydroxybenzaldehyde + NADPH + H+ 7.1% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis 3-hydroxybenzyl alcohol + NADP+
-
 ?
3-nitrobenzaldehyde + NADPH + H+ 7.0% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis 3-nitrobenzyl alcohol + NADP+
-
 ?
ethyl 2-chloro-3-oxobutanoate + NADPH + H+ 135.7% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis (S)-ethyl 2-chloro-3-hydroxybutanoate + NADP+
-
 ?
ethyl 3-oxobutanoate + NADPH + H+ 25.2% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis ethyl 3-hydroxybutanoate + NADP+
-
 ?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
-
 Scheffersomyces stipitis (S)-ethyl 4-chloro-3-hydroxybutanoate + NADP+
-
 ?
isatin + NADPH + H+ 17.7% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis 3-hydroxy-2-oxoindole + NADP+
-
 ?
methyl 2-chloro-3-oxobutanoate + NADPH + H+ 25.1% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis (S)-methyl 2-chloro-3-hydroxybutanoate + NADP+
-
 ?
methyl 4-chloro-3-oxobutanoate + NADPH + H+ 25.4% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis (S)-methyl 4-chloro-3-hydroxybutanoate + NADP+
-
 ?
additional information no activity for methyl-3-oxobutanoate and simple ketones and aldehydes such as benzaldehyde and acrolein. No substrate: pyridine-4-aldehyde, ethyl 4,4,4-trifluoro-3-oxobutanoate, pyridine-3-aldehyde or p-nitrobenzaldehyde Scheffersomyces stipitis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Scheffersomyces stipitis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Scheffersomyces stipitis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 sharp decrease in activity above 40°C Scheffersomyces stipitis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 stable for 60 min Scheffersomyces stipitis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Scheffersomyces stipitis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 6.5 more than 90% of maximum activity Scheffersomyces stipitis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 7.5 4°C, maintains more than 80% of maximum activity for 48 h Scheffersomyces stipitis

Cofactor

Cofactor Comment Organism Structure
NADPH highly specific for NADPH as cofactor Scheffersomyces stipitis