Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.184 extracted from

  • Suwa, Y.; Ohtsuka, J.; Miyakawa, T.; Imai, F.L.; Okai, M.; Sawano, Y.; Yasohara, Y.; Kataoka, M.; Shimizu, S.; Tanokura, M.
    Expression, purification, crystallization and preliminary X-ray analysis of carbonyl reductase S1 from Candida magnoliae (2012), Acta Crystallogr. Sect. F, 68, 540-542.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.184

Application

Application Comment Organism
synthesis enzyme product ethyl (S)-4-chloro-3-hydroxybutanoate is a chiral compound valuable as a building block for pharmaceuticals Starmerella magnoliae

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) Starmerella magnoliae

Crystallization (Commentary)

Crystallization (Comment) Organism
purified detagged recombinant enzyme, sitting-drop vapour-diffusion method, protein solution is supplemented with 5 mM beta-NADPH, crystallization from 31% v/v PEG 400, 0.2 M Li2SO4, 0.08 M sodium potassium tartrate tetrahydrate, 0.1 M sodium cacodylate, pH 6.5, at 20°C, 3 days, X-ray diffraction structure determination and analysis at 1.90 A resolution Starmerella magnoliae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ Starmerella magnoliae
-
 ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
 ?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ Starmerella magnoliae AKU4643
-
 ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Starmerella magnoliae Q9C4B3
-
-
Starmerella magnoliae AKU4643 Q9C4B3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity and anion exchange chromatography, cleavage of the tag by thrombin protease, and gel filtration Starmerella magnoliae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
-
 Starmerella magnoliae ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
 ?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
-
 Starmerella magnoliae AKU4643 ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
carbonyl reductase S1
-
 Starmerella magnoliae
NADPH-dependent carbonyl reductase S1
-
 Starmerella magnoliae

Cofactor

Cofactor Comment Organism Structure
NADPH strict NADPH-dependence Starmerella magnoliae

General Information

General Information Comment Organism
evolution the enzyme is a member of the short-chain dehydrogenase/reductase (SDR) family Starmerella magnoliae