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Literature summary for 1.1.1.184 extracted from
- Studies on reduction of S-nitrosoglutathione by human carbonyl reductases 1 and 3 (2011), Chem. Biol. Interact., 191, 95-103.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C227S | displays a similar kcat, but a 30fold higher Km value for S-nitrosoglutathione, and does not show substrate inhibition | Homo sapiens |
| D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P | mutations introduce activity towards S-nitrosoglutathione | Homo sapiens |
| P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P | mutations introduce activity towards S-nitrosoglutathione | Homo sapiens |
| Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S | mutations introduce activity towards S-nitrosoglutathione | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| S-nitrosoglutathione | substrate inhibition above 5 * Km, enzyme is covalantly modified. Treatment with dithiothreitol, but not with ascorbic acid, rescues the activity | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0128 | - |
9,10-phenanthrenequinone | mutant C227S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 0.028 | - |
S-nitrosoglutathione | wild-type, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 0.0354 | - |
9,10-phenanthrenequinone | wild-type, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 0.81 | - |
S-nitrosoglutathione | mutant C227S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 0.832 | - |
S-nitrosoglutathione | mutant Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 1.09 | - |
S-nitrosoglutathione | mutant P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244PpH 7.4, temperature not specified in the publication | Homo sapiens | |
| 2.93 | - |
S-nitrosoglutathione | mutant D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, pH 7.4, temperature not specified in the publication | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O75828 | isoform CBR 3 | - |
| Homo sapiens | P16152 | isoform CBR 1 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 9,10-phenanthrenequinone + NADPH + H+ | - |
Homo sapiens | ? + NADP+ | - |
? | |
| S-nitrosoglutathione + NADPH + H+ | - |
Homo sapiens | ? + NADP+ | - |
? | |
| S-nitrosoglutathione + NADPH + H+ | no substrate for wild-type, but substrate for mutants D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, and Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S | Homo sapiens | ? + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CBR 1 | - |
Homo sapiens |
| CBR 3 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.02 | - |
S-nitrosoglutathione | mutant C227S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 2.62 | - |
S-nitrosoglutathione | wild-type, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 5.05 | - |
S-nitrosoglutathione | mutant D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 5.25 | - |
9,10-phenanthrenequinone | wild-type, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 10.83 | - |
S-nitrosoglutathione | mutant P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244PpH 7.4, temperature not specified in the publication | Homo sapiens | |
| 20.45 | - |
S-nitrosoglutathione | mutant Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 36.2 | - |
9,10-phenanthrenequinone | mutant C227S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.67 | - |
S-nitrosoglutathione | wild-type, pH 7.4, temperature not specified in the publication | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.72 | - |
S-nitrosoglutathione | mutant D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 2.5 | - |
S-nitrosoglutathione | mutant C227S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 9.93 | - |
S-nitrosoglutathione | mutant P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244PpH 7.4, temperature not specified in the publication | Homo sapiens | |
| 24.58 | - |
S-nitrosoglutathione | mutant Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 93.45 | - |
S-nitrosoglutathione | wild-type, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 148.3 | - |
9,10-phenanthrenequinone | wild-type, pH 7.4, temperature not specified in the publication | Homo sapiens | |
| 2828 | - |
9,10-phenanthrenequinone | mutant C227S, pH 7.4, temperature not specified in the publication | Homo sapiens | |
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