Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aeropyrum pernix |
Crystallization (Comment) | Organism |
---|---|
three-dimensional strcuture determined by molecular replacement at a resolution of 2.09 A, in the presence of NADPH. Extensive formation of the intrasubunit ion pair networks, and the presence of the strong intersubunit interaction, is likely responsible for thermostability | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0145 | - |
9,10-phenanthrenequinone | pH 6.0, 50°C | Aeropyrum pernix | |
0.116 | - |
NADPH | pH 6.0, 50°C | Aeropyrum pernix | |
0.176 | - |
p-toluquinone | pH 6.0, 50°C | Aeropyrum pernix | |
0.7 | - |
ethyl 4-chloro-3-oxobutanoate | pH 6.0, 50°C | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9Y8Y1 | cf. EC 1.1.1.100 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9,10-phenanthrenequinone + NADPH + H+ | - |
Aeropyrum pernix | ? + NADP+ | - |
? | |
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ | - |
Aeropyrum pernix | ethyl (3S)-4-chloro-3-hydroxybutanoate + NADP+ | - |
? | |
isatin + NADPH + H+ | - |
Aeropyrum pernix | 3-hydroxy-2-oxoindole + NADP+ | - |
? | |
p-toluquinone + NADPH | - |
Aeropyrum pernix | ? + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
above 70°C | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
10 min, 75% residual activity | Aeropyrum pernix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.05 | - |
ethyl 4-chloro-3-oxobutanoate | pH 6.0, 50°C | Aeropyrum pernix | |
1.27 | - |
9,10-phenanthrenequinone | pH 6.0, 50°C | Aeropyrum pernix | |
2.5 | - |
p-toluquinone | pH 6.0, 50°C | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Aeropyrum pernix |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 11.5 | - |
Aeropyrum pernix |