Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.188 extracted from

  • Barski, O.A.; Watanabe, K.
    Kinetic mechanism of ketoreductase activity of prostaglandin F synthase from bovine lung (1993), FEBS Lett., 320, 107-110.
    View publication on PubMed
Search for more literature for 1.1.1.188

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
 NADPH
-
 Bos taurus
0.174
-
 Prostaglandin D2
-
 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 two isozymes, lung-type and liver-type
-

Source Tissue

Source Tissue Comment Organism Textmining
lung two different active sites Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
 Bos taurus (5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?
4-nitrobenzaldehyde + NADPH
-
 Bos taurus 4-nitrobenzyl alcohol + NADP+
-
 ?
9,10-phenanthrenequinone + NADPH
-
 Bos taurus ?
-
 ?
menadione + NADPH
-
 Bos taurus ?
-
 ?
additional information two different active sites, one for the endoperoxide group of prostaglandin H2 and the other one for the keto group of prostaglandin D2 and other carbonyl compounds Bos taurus ?
-
 ?
prostaglandin H2 + NADPH + H+
-
 Bos taurus (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Bos taurus