Cloned (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D57A | about 4fold increase in catalytic efficiency | Arabidopsis thaliana |
H52A | about 40% decrease in catalytic efficiency | Arabidopsis thaliana |
T49A | less than 0.5% of wild-type activity. Thermodynamic data indicate a negative enthalpic change, as well as a significant decrease in binding affinity with NADPH. Residue Thr49 is essential for overall catalytic conversion | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
4-coumaraldehyde | wild-type, 30°C, pH not specified in the publication | Arabidopsis thaliana | |
0.036 | - |
4-coumaraldehyde | mutant D57A, 30°C, pH not specified in the publication | Arabidopsis thaliana | |
0.114 | - |
4-coumaraldehyde | mutant T49A, 30°C, pH not specified in the publication | Arabidopsis thaliana | |
0.117 | - |
4-coumaraldehyde | mutant H52A, 30°C, pH not specified in the publication | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O49482 | isoform CAD5 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaraldehyde + NADPH + H+ | - |
Arabidopsis thaliana | 4-coumaryl alcohol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CAD5 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
4-coumaraldehyde | mutant T49A, 30°C, pH not specified in the publication | Arabidopsis thaliana | |
6.8 | - |
4-coumaraldehyde | wild-type, 30°C, pH not specified in the publication | Arabidopsis thaliana | |
40.2 | - |
4-coumaraldehyde | mutant H52A, 30°C, pH not specified in the publication | Arabidopsis thaliana | |
74.2 | - |
4-coumaraldehyde | mutant D57A, 30°C, pH not specified in the publication | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
4-coumaraldehyde | mutant T49A, 30°C, pH not specified in the publication | Arabidopsis thaliana | |
345 | - |
4-coumaraldehyde | mutant H52A, 30°C, pH not specified in the publication | Arabidopsis thaliana | |
523 | - |
4-coumaraldehyde | wild-type, 30°C, pH not specified in the publication | Arabidopsis thaliana | |
2061 | - |
4-coumaraldehyde | mutant D57A, 30°C, pH not specified in the publication | Arabidopsis thaliana |