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Literature summary for 1.1.1.195 extracted from

  • Lee, C.; Bedgar, D.L.; Davin, L.B.; Lewis, N.G.
    Assessment of a putative proton relay in Arabidopsis cinnamyl alcohol dehydrogenase catalysis (2013), Org. Biomol. Chem., 11, 1127-1134.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
D57A about 4fold increase in catalytic efficiency Arabidopsis thaliana
H52A about 40% decrease in catalytic efficiency Arabidopsis thaliana
T49A less than 0.5% of wild-type activity. Thermodynamic data indicate a negative enthalpic change, as well as a significant decrease in binding affinity with NADPH. Residue Thr49 is essential for overall catalytic conversion Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
4-coumaraldehyde wild-type, 30°C, pH not specified in the publication Arabidopsis thaliana
0.036
-
4-coumaraldehyde mutant D57A, 30°C, pH not specified in the publication Arabidopsis thaliana
0.114
-
4-coumaraldehyde mutant T49A, 30°C, pH not specified in the publication Arabidopsis thaliana
0.117
-
4-coumaraldehyde mutant H52A, 30°C, pH not specified in the publication Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O49482 isoform CAD5
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaraldehyde + NADPH + H+
-
Arabidopsis thaliana 4-coumaryl alcohol + NADP+
-
?

Synonyms

Synonyms Comment Organism
CAD5
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.026
-
4-coumaraldehyde mutant T49A, 30°C, pH not specified in the publication Arabidopsis thaliana
6.8
-
4-coumaraldehyde wild-type, 30°C, pH not specified in the publication Arabidopsis thaliana
40.2
-
4-coumaraldehyde mutant H52A, 30°C, pH not specified in the publication Arabidopsis thaliana
74.2
-
4-coumaraldehyde mutant D57A, 30°C, pH not specified in the publication Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.23
-
4-coumaraldehyde mutant T49A, 30°C, pH not specified in the publication Arabidopsis thaliana
345
-
4-coumaraldehyde mutant H52A, 30°C, pH not specified in the publication Arabidopsis thaliana
523
-
4-coumaraldehyde wild-type, 30°C, pH not specified in the publication Arabidopsis thaliana
2061
-
4-coumaraldehyde mutant D57A, 30°C, pH not specified in the publication Arabidopsis thaliana