Application | Comment | Organism |
---|---|---|
analysis | the enzyme is utilized in a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview | Levilactobacillus brevis |
analysis | the enzyme is utilized in a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview | Thermoanaerobacter brockii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | evaluation of a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview | Levilactobacillus brevis |
additional information | evaluation of a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview | Thermoanaerobacter brockii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
12-ketochenodeoxycholic acid | inhibition of the ADH from Lactobacillus brevis by bile acids, acetone prevents partially | Levilactobacillus brevis | |
acetone | at high concentrations of e.g. 3.4 M | Levilactobacillus brevis | |
acetone | at high concentrations of e.g. 3.4 M | Thermoanaerobacter brockii | |
cholic acid | inhibition of the ADH from Lactobacillus brevis by bile acids, acetone prevents partially | Levilactobacillus brevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-propanol + NADP+ | Levilactobacillus brevis | - |
acetone + NADPH | - |
r | |
2-propanol + NADP+ | Thermoanaerobacter brockii | - |
acetone + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Levilactobacillus brevis | - |
- |
- |
Thermoanaerobacter brockii | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Levilactobacillus brevis | - |
commercial preparation | - |
Thermoanaerobacter brockii | - |
Storage Stability | Organism |
---|---|
1 unit of ADH dissolved in 0.5 ml of 0.1 M potassium phosphate buffer, pH 8.0, containing 4% w/v cholic acid 100 mM, 100 mM NADP+, 1 mM dithiothreitol, and 10% or 25% v/v acetone, 23 days, 51% and 46% residual activity, respectively | Levilactobacillus brevis |
1 unit of ADH dissolved in 0.5 ml of 0.1 M potassium phosphate buffer, pH 8.0, containing 4% w/v cholic acid 100 mM, 100 mM NADP+, 1 mM dithiothreitol, and 10% or 25% v/v acetone, 23 days, quite stable | Thermoanaerobacter brockii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-propanol + NADP+ | - |
Levilactobacillus brevis | acetone + NADPH | - |
r | |
2-propanol + NADP+ | - |
Thermoanaerobacter brockii | acetone + NADPH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Levilactobacillus brevis |
ADH | - |
Thermoanaerobacter brockii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Levilactobacillus brevis |
22 | - |
assay at room temperature | Thermoanaerobacter brockii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Levilactobacillus brevis |
8 | - |
assay at | Thermoanaerobacter brockii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent on | Levilactobacillus brevis | |
NADP+ | dependent on | Thermoanaerobacter brockii | |
NADPH | dependent on | Levilactobacillus brevis | |
NADPH | dependent on | Thermoanaerobacter brockii |