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Literature summary for 1.1.1.2 extracted from

  • Goihberg, E.; Dym, O.; Tel-Or, S.; Shimon, L.; Frolow, F.; Peretz, M.; Burstein, Y.
    Thermal stabilization of the protozoan Entamoeba histolytica alcohol dehydrogenase by a single proline substitution (2008), Proteins, 72, 711-719.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, wild-type and mutant enzyme Thermoanaerobacter brockii
expression in Escherichia coli, wild-type and mutant enzyme Entamoeba histolytica

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of D275P–EhADH1 are grown using the hanging-drop vapor-diffusion method at 20°C. Crystal structure of the thermostabilized mutant D275P-EhADH1 suggests that a proline residue at position 275 thermostabilizes the enzymes by reducing flexibility and by reinforcing hydrophobic interactions at the dimer–dimer interface of the tetrameric ADH Entamoeba histolytica
crystals of P275D–TbADH1 are grown using the hanging-drop vapor-diffusion method at 20°C. Crystal structure of the thermostabilized mutant P275D-EhADH1 suggests that a proline residue at position 275 thermostabilizes the enzymes by reducing flexibility and by reinforcing hydrophobic interactions at the dimer–dimer interface of the tetrameric ADH Thermoanaerobacter brockii

Protein Variants

Protein Variants Comment Organism
D275P mutation significantly enhances the thermal stability of EhADH1 Entamoeba histolytica
P275D mutation reduces the thermostability of the enzyme Thermoanaerobacter brockii

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica P35630
-
-
Thermoanaerobacter brockii P14941
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzyme Thermoanaerobacter brockii
wild-type and mutant enzyme Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-butanol + NADP+
-
Thermoanaerobacter brockii ?
-
?
2-butanol + NADP+
-
Entamoeba histolytica ?
-
?

Synonyms

Synonyms Comment Organism
EhADH1
-
Entamoeba histolytica
TbADH1
-
Thermoanaerobacter brockii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
76
-
1 h, 50% loss of activity of wild-type enzyme Thermoanaerobacter brockii
76
-
1 h, 50% loss of activity of wild-type enzyme Entamoeba histolytica
87
-
1 h, 50% loss of activity of mutant enzyme D275P Thermoanaerobacter brockii
87
-
1 h, 50% loss of activity of mutant enzyme D275P Entamoeba histolytica