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Literature summary for 1.1.1.2 extracted from

  • Ma, K.; Adams, M.W.
    An unusual oxygen-sensitive, iron- and zinc-containing alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus (1999), J. Bacteriol., 181, 1163-1170.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.071
-
NADPH 80°C, pH not specified in the publication Pyrococcus furiosus
0.071
-
NADP+ 80°C, pH not specified in the publication Pyrococcus furiosus
0.091
-
phenylacetaldehyde 80°C, pH not specified in the publication Pyrococcus furiosus
0.17
-
acetaldehyde 80°C, pH not specified in the publication Pyrococcus furiosus
0.2
-
methylglyoxal 80°C, pH not specified in the publication Pyrococcus furiosus
20
-
NAD+ 80°C, pH not specified in the publication Pyrococcus furiosus
29.4
-
ethanol 80°C, pH not specified in the publication Pyrococcus furiosus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Pyrococcus furiosus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains 1 gatom iron per subunit. The iron is present as a low-spin Fe(II) Pyrococcus furiosus
Zinc contains 1 gatom per subunit Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44840
-
6 * 44840, mass spectrometric analysis Pyrococcus furiosus
48000
-
6 * 48000, SDS-PAGE Pyrococcus furiosus
270000
-
gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetaldehyde + NADPH + H+ Pyrococcus furiosus the proposed physiological role of NADP-dependent alcohol dehydrogenase in Pyrococcus furiosus is to both dispose of excess reducing equivalents and to detoxify the aldehydes produced by the fermentative pathways ethanol + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Oxidation Stability

Oxidation Stability Organism
oxygen sensitive, half-life in air is about 1 h at 23°C. It is stable under anaerobic conditions even at high temperature, with half-lives at 85 and 95°C of 160 and 7 h, respectively Pyrococcus furiosus

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
72.3
-
80°C, pH not specified in the publication Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + NADP+ 169% of the activity compared to ethanol Pyrococcus furiosus butanal + NADPH + H+
-
r
1-heptanol + NADP+ 86% of the activity compared to ethanol Pyrococcus furiosus heptanal + NADPH + H+
-
r
1-hexanol + NADP+ 95% of the activity compared to ethanol Pyrococcus furiosus hexanal + NADPH + H+
-
r
1-octanol + NADP+ 57% of the activity compared to ethanol Pyrococcus furiosus octanal + NADPH + H+
-
r
1-pentanol + NADP+ 172% of the activity compared to ethanol Pyrococcus furiosus pentanal + NADPH + H+
-
r
1-propanol + NADP+ 156% of the activity compared to ethanol Pyrococcus furiosus propanal + NADPH + H+
-
r
2-phenylethanol + NADP+ 76% of the activity compared to ethanol Pyrococcus furiosus phenylacetaldehyde + NADPH + H+
-
r
acetaldehyde + NADPH + H+
-
Pyrococcus furiosus ethanol + NADP+
-
r
acetaldehyde + NADPH + H+ the proposed physiological role of NADP-dependent alcohol dehydrogenase in Pyrococcus furiosus is to both dispose of excess reducing equivalents and to detoxify the aldehydes produced by the fermentative pathways Pyrococcus furiosus ethanol + NADP+
-
?
ethanol + NAD+ kcat/Km for NAD+ is 1900fold lower compared to NADP+ Pyrococcus furiosus acetaldehyde + NADH + H+
-
r
ethanol + NADP+ kcat/Km for NAD+ is 1900fold lower compared to NADP+ Pyrococcus furiosus acetaldehyde + NADPH + H+
-
r
isobutanol + NADP+ 122% of the activity compared to ethanol Pyrococcus furiosus isobutanal + NADPH + H+
-
r
methylglyoxal + NADPH 27% of the activity compared to ethanol Pyrococcus furiosus ?
-
?
additional information no activity with methanol, 2-propanol, glycerol, D-sorbitol Pyrococcus furiosus ?
-
?

Subunits

Subunits Comment Organism
homohexamer 6 * 48000, SDS-PAGE Pyrococcus furiosus
homohexamer 6 * 44840, mass spectrometric analysis Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
above Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
23
-
anaerobic conditions, 24 h, stable Pyrococcus furiosus
85
-
anaerobic conditions, half-life: 160 h Pyrococcus furiosus
95
-
anaerobic conditions, half-life: 7 h Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4
-
NAD+ 80°C, pH not specified in the publication Pyrococcus furiosus
3.7
-
methylglyoxal 80°C, pH not specified in the publication Pyrococcus furiosus
5.5
-
acetaldehyde 80°C, pH not specified in the publication Pyrococcus furiosus
6.3
-
NADPH 80°C, pH not specified in the publication Pyrococcus furiosus
10.4
-
phenylacetaldehyde 80°C, pH not specified in the publication Pyrococcus furiosus
15.8
-
NADP+ 80°C, pH not specified in the publication Pyrococcus furiosus
19.2
-
ethanol 80°C, pH not specified in the publication Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4 10.2 80°C, optimal pH for ethanol oxidation Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
NADP+ kcat/Km for NAD+ is 1900fold lower compared to NADP+ Pyrococcus furiosus
NADPH
-
Pyrococcus furiosus

General Information

General Information Comment Organism
physiological function the proposed physiological role of NADP-dependent alcohol dehydrogenase in Pyrococcus furiosus is to both dispose of excess reducing equivalents and to detoxify the aldehydes produced by the fermentative pathways Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0028
-
acetaldehyde 80°C, pH not specified in the publication Pyrococcus furiosus
0.12
-
NAD+ 80°C, pH not specified in the publication Pyrococcus furiosus
0.65
-
ethanol 80°C, pH not specified in the publication Pyrococcus furiosus
18.5
-
methylglyoxal 80°C, pH not specified in the publication Pyrococcus furiosus
93
-
NADPH 80°C, pH not specified in the publication Pyrococcus furiosus
114
-
phenylacetaldehyde 80°C, pH not specified in the publication Pyrococcus furiosus
223
-
NADP+ 80°C, pH not specified in the publication Pyrococcus furiosus