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Literature summary for 1.1.1.2 extracted from

  • Ma, M.; Wang, X.; Zhang, X.; Zhao, X.
    Alcohol dehydrogenases from Scheffersomyces stipitis involved in the detoxification of aldehyde inhibitors derived from lignocellulosic biomass conversion (2013), Appl. Microbiol. Biotechnol., 97, 8411-8425.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Scheffersomyces stipitis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
NADPH substrate furfural, pH 7.2, 30°C Scheffersomyces stipitis
0.26
-
NADPH substrate 5-hydroxymethylfurfural, pH 7.2, 30°C Scheffersomyces stipitis
4.11
-
NADPH substrate furfural, pH 7.2, 30°C Scheffersomyces stipitis
20.55
-
NADH substrate furfural, pH 7.2, 30°C Scheffersomyces stipitis
23.84
-
NADH substrate furfural, pH 7.2, 30°C Scheffersomyces stipitis

Organism

Organism UniProt Comment Textmining
Scheffersomyces stipitis A3GHL4
-
-
Scheffersomyces stipitis A3GHR6
-
-
Scheffersomyces stipitis ATCC 58785 A3GHL4
-
-
Scheffersomyces stipitis ATCC 58785 A3GHR6
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11
-
substrate formaldehyde, cofactor NADH, pH 7.2, 30°C Scheffersomyces stipitis
1.27
-
substrate formaldehyde, cofactor NADPH, pH 7.2, 30°C Scheffersomyces stipitis
12.22
-
substrate acetaldehyde, cofactor NADPH, pH 7.2, 30°C Scheffersomyces stipitis
15.56
-
substrate acetaldehyde, cofactor NADH, pH 7.2, 30°C Scheffersomyces stipitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxymethylfurfural + NADPH + H+
-
Scheffersomyces stipitis 5-hydroxymethylfurfuryl alcohol + NADP+
-
?
5-hydroxymethylfurfural + NADPH + H+
-
Scheffersomyces stipitis ATCC 58785 5-hydroxymethylfurfuryl alcohol + NADP+
-
?
acetaldehyde + NADPH + H+
-
Scheffersomyces stipitis ethanol + NADP+
-
?
acetaldehyde + NADPH + H+
-
Scheffersomyces stipitis ATCC 58785 ethanol + NADP+
-
?
formaldehyde + NADPH + H+
-
Scheffersomyces stipitis methanol + NADP+
-
?
formaldehyde + NADPH + H+
-
Scheffersomyces stipitis ATCC 58785 methanol + NADP+
-
?
furfural + NADH + H+
-
Scheffersomyces stipitis furfuryl alcohol + NAD+
-
?
furfural + NADH + H+
-
Scheffersomyces stipitis ATCC 58785 furfuryl alcohol + NAD+
-
?
furfural + NADPH + H+
-
Scheffersomyces stipitis furfuryl alcohol + NADP+
-
?
furfural + NADPH + H+
-
Scheffersomyces stipitis ATCC 58785 furfuryl alcohol + NADP+
-
?

Synonyms

Synonyms Comment Organism
ADH4
-
Scheffersomyces stipitis
Adh6
-
Scheffersomyces stipitis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Scheffersomyces stipitis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.21
-
NADPH substrate furfural, pH 7.2, 30°C Scheffersomyces stipitis
2.7
-
NADH substrate furfural, pH 7.2, 30°C Scheffersomyces stipitis
4.75
-
NADPH substrate furfural, pH 7.2, 30°C Scheffersomyces stipitis
6.23
-
NADH substrate furfural, pH 7.2, 30°C Scheffersomyces stipitis
6.5
-
NADPH substrate 5-hydroxymethylfurfural, pH 7.2, 30°C Scheffersomyces stipitis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Scheffersomyces stipitis

Cofactor

Cofactor Comment Organism Structure
NAD+ NADP+ is preferred over NAD+ Scheffersomyces stipitis
NADH NADPH is preferred over NADH Scheffersomyces stipitis
NADP+ NADP+ is preferred over NAD+ Scheffersomyces stipitis
NADPH NADPH is preferred over NADH Scheffersomyces stipitis

Expression

Organism Comment Expression
Scheffersomyces stipitis transcription levels of isoform ADH4 display more than 100fold increase in response to furfural and 150fold increase in response to hydroxymethylfurfural, respectively up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.13
-
NADH substrate furfural, pH 7.2, 30°C Scheffersomyces stipitis
0.26
-
NADH substrate furfural, pH 7.2, 30°C Scheffersomyces stipitis
0.54
-
NADPH substrate furfural, pH 7.2, 30°C Scheffersomyces stipitis
19.9
-
NADPH substrate furfural, pH 7.2, 30°C Scheffersomyces stipitis
25.3
-
NADPH substrate 5-hydroxymethylfurfural, pH 7.2, 30°C Scheffersomyces stipitis