Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Scheffersomyces stipitis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
NADPH | substrate furfural, pH 7.2, 30°C | Scheffersomyces stipitis | |
0.26 | - |
NADPH | substrate 5-hydroxymethylfurfural, pH 7.2, 30°C | Scheffersomyces stipitis | |
4.11 | - |
NADPH | substrate furfural, pH 7.2, 30°C | Scheffersomyces stipitis | |
20.55 | - |
NADH | substrate furfural, pH 7.2, 30°C | Scheffersomyces stipitis | |
23.84 | - |
NADH | substrate furfural, pH 7.2, 30°C | Scheffersomyces stipitis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scheffersomyces stipitis | A3GHL4 | - |
- |
Scheffersomyces stipitis | A3GHR6 | - |
- |
Scheffersomyces stipitis ATCC 58785 | A3GHL4 | - |
- |
Scheffersomyces stipitis ATCC 58785 | A3GHR6 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.11 | - |
substrate formaldehyde, cofactor NADH, pH 7.2, 30°C | Scheffersomyces stipitis |
1.27 | - |
substrate formaldehyde, cofactor NADPH, pH 7.2, 30°C | Scheffersomyces stipitis |
12.22 | - |
substrate acetaldehyde, cofactor NADPH, pH 7.2, 30°C | Scheffersomyces stipitis |
15.56 | - |
substrate acetaldehyde, cofactor NADH, pH 7.2, 30°C | Scheffersomyces stipitis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxymethylfurfural + NADPH + H+ | - |
Scheffersomyces stipitis | 5-hydroxymethylfurfuryl alcohol + NADP+ | - |
? | |
5-hydroxymethylfurfural + NADPH + H+ | - |
Scheffersomyces stipitis ATCC 58785 | 5-hydroxymethylfurfuryl alcohol + NADP+ | - |
? | |
acetaldehyde + NADPH + H+ | - |
Scheffersomyces stipitis | ethanol + NADP+ | - |
? | |
acetaldehyde + NADPH + H+ | - |
Scheffersomyces stipitis ATCC 58785 | ethanol + NADP+ | - |
? | |
formaldehyde + NADPH + H+ | - |
Scheffersomyces stipitis | methanol + NADP+ | - |
? | |
formaldehyde + NADPH + H+ | - |
Scheffersomyces stipitis ATCC 58785 | methanol + NADP+ | - |
? | |
furfural + NADH + H+ | - |
Scheffersomyces stipitis | furfuryl alcohol + NAD+ | - |
? | |
furfural + NADH + H+ | - |
Scheffersomyces stipitis ATCC 58785 | furfuryl alcohol + NAD+ | - |
? | |
furfural + NADPH + H+ | - |
Scheffersomyces stipitis | furfuryl alcohol + NADP+ | - |
? | |
furfural + NADPH + H+ | - |
Scheffersomyces stipitis ATCC 58785 | furfuryl alcohol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH4 | - |
Scheffersomyces stipitis |
Adh6 | - |
Scheffersomyces stipitis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Scheffersomyces stipitis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.21 | - |
NADPH | substrate furfural, pH 7.2, 30°C | Scheffersomyces stipitis | |
2.7 | - |
NADH | substrate furfural, pH 7.2, 30°C | Scheffersomyces stipitis | |
4.75 | - |
NADPH | substrate furfural, pH 7.2, 30°C | Scheffersomyces stipitis | |
6.23 | - |
NADH | substrate furfural, pH 7.2, 30°C | Scheffersomyces stipitis | |
6.5 | - |
NADPH | substrate 5-hydroxymethylfurfural, pH 7.2, 30°C | Scheffersomyces stipitis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Scheffersomyces stipitis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NADP+ is preferred over NAD+ | Scheffersomyces stipitis | |
NADH | NADPH is preferred over NADH | Scheffersomyces stipitis | |
NADP+ | NADP+ is preferred over NAD+ | Scheffersomyces stipitis | |
NADPH | NADPH is preferred over NADH | Scheffersomyces stipitis |
Organism | Comment | Expression |
---|---|---|
Scheffersomyces stipitis | transcription levels of isoform ADH4 display more than 100fold increase in response to furfural and 150fold increase in response to hydroxymethylfurfural, respectively | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
NADH | substrate furfural, pH 7.2, 30°C | Scheffersomyces stipitis | |
0.26 | - |
NADH | substrate furfural, pH 7.2, 30°C | Scheffersomyces stipitis | |
0.54 | - |
NADPH | substrate furfural, pH 7.2, 30°C | Scheffersomyces stipitis | |
19.9 | - |
NADPH | substrate furfural, pH 7.2, 30°C | Scheffersomyces stipitis | |
25.3 | - |
NADPH | substrate 5-hydroxymethylfurfural, pH 7.2, 30°C | Scheffersomyces stipitis |