Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 2.5 mM, in presence of 50 mM substrate, 12% decrease in activity | Malus domestica | |
CuCl2 | - |
Malus domestica | |
MgCl2 | 2.5 mM, in presence of 50 mM substrate, 12% decrease in activity | Malus domestica | |
phosphate | mixed-type inhibition | Malus domestica | |
ZnCl2 | 2 mM, 58% inhibition, partially reversible by EDTA | Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
D-sorbitol-6-phosphate | pH 9.0 | Malus domestica | |
3 | - |
D-sorbitol-6-phosphate | pH 9.0 | Malus domestica | |
5.1 | - |
D-Glucose-6-phosphate | pH 9.0, presence of 2.5 mM MgCl2 | Malus domestica | |
9.8 | - |
D-Glucose-6-phosphate | pH 9.0 | Malus domestica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 2.5 mM, in presence of 5 mM substrate, 40% increase in activity | Malus domestica | |
MgCl2 | 2.5 mM, in presence of 5 mM substrate, 52% increase in activity | Malus domestica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33000, SDS-PAGE | Malus domestica |
68000 | - |
gel filtration | Malus domestica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
Borkh. cv. Gala | - |
Purification (Comment) | Organism |
---|---|
- |
Malus domestica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | fully developed | Malus domestica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.94 | - |
pH 9.0 | Malus domestica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADPH | - |
Malus domestica | D-sorbitol 6-phosphate + NADP+ | - |
r | |
D-sorbitol 6-phosphate + NADP+ | - |
Malus domestica | D-glucose 6-phosphate + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33000, SDS-PAGE | Malus domestica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Malus domestica | |
NADPH | - |
Malus domestica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
phosphate | pH 9.0 | Malus domestica |