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Literature summary for 1.1.1.200 extracted from

  • Zhou, R.; Sicher, R.C.; Cheng, L.; Quebedeaux, B.
    Regulation of apple leaf aldose-6-phosphate reductase activity by inorganic phosphate and divalent cations (2003), Funct. Plant Biol., 30, 1037-1043.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 2.5 mM, in presence of 50 mM substrate, 12% decrease in activity Malus domestica
CuCl2
-
Malus domestica
MgCl2 2.5 mM, in presence of 50 mM substrate, 12% decrease in activity Malus domestica
phosphate mixed-type inhibition Malus domestica
ZnCl2 2 mM, 58% inhibition, partially reversible by EDTA Malus domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
D-sorbitol-6-phosphate pH 9.0 Malus domestica
3
-
D-sorbitol-6-phosphate pH 9.0 Malus domestica
5.1
-
D-Glucose-6-phosphate pH 9.0, presence of 2.5 mM MgCl2 Malus domestica
9.8
-
D-Glucose-6-phosphate pH 9.0 Malus domestica

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 2.5 mM, in presence of 5 mM substrate, 40% increase in activity Malus domestica
MgCl2 2.5 mM, in presence of 5 mM substrate, 52% increase in activity Malus domestica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
2 * 33000, SDS-PAGE Malus domestica
68000
-
gel filtration Malus domestica

Organism

Organism UniProt Comment Textmining
Malus domestica
-
Borkh. cv. Gala
-

Purification (Commentary)

Purification (Comment) Organism
-
Malus domestica

Source Tissue

Source Tissue Comment Organism Textmining
leaf fully developed Malus domestica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.94
-
pH 9.0 Malus domestica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + NADPH
-
Malus domestica D-sorbitol 6-phosphate + NADP+
-
r
D-sorbitol 6-phosphate + NADP+
-
Malus domestica D-glucose 6-phosphate + NADPH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 33000, SDS-PAGE Malus domestica

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Malus domestica
NADPH
-
Malus domestica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.42
-
phosphate pH 9.0 Malus domestica