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Literature summary for 1.1.1.201 extracted from

  • Liu, L.; Aigner, A.; Schmid, R.D.
    Identification, cloning, heterologous expression, and characterization of a NADPH-dependent 7beta-hydroxysteroid dehydrogenase from Collinsella aerofaciens (2011), Appl. Microbiol. Biotechnol., 90, 127-135.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Collinsella aerofaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
NADPH pH 8.0, 25°C Collinsella aerofaciens
0.0052
-
7-ketolithocholic acid pH 8.0, 25°C Collinsella aerofaciens
0.0053
-
NADP+ pH 8.0, 25°C Collinsella aerofaciens
0.0062
-
Ursodeoxycholic acid pH 8.0, 25°C Collinsella aerofaciens
0.0092
-
Dehydrocholic acid pH 8.0, 25°C Collinsella aerofaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
2 * 30000, SDS-PAGE Collinsella aerofaciens
56100
-
gel filtration Collinsella aerofaciens

Organism

Organism UniProt Comment Textmining
Collinsella aerofaciens
-
-
-
Collinsella aerofaciens DSM 3979
-
-
-

Storage Stability

Storage Stability Organism
-20°C, 50 mM potassium phosphate, pH 8.0, stable for at least 3 months with several times of freezing and thawing Collinsella aerofaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-ketolithocholic acid + NADPH + H+
-
Collinsella aerofaciens ursodeoxycholic acid + NADP+ only product, 90% conversion, 71% yield r
7-ketolithocholic acid + NADPH + H+
-
Collinsella aerofaciens DSM 3979 ursodeoxycholic acid + NADP+ only product, 90% conversion, 71% yield r
dehydrocholic acid + NADPH + H+
-
Collinsella aerofaciens ? + NADP+ + H+
-
?
dehydrocholic acid + NADPH + H+
-
Collinsella aerofaciens DSM 3979 ? + NADP+ + H+
-
?
ursodeoxycholic acid + NADP+
-
Collinsella aerofaciens 7-ketolithocholic acid + NADPH + H+
-
r
ursodeoxycholic acid + NADP+
-
Collinsella aerofaciens DSM 3979 7-ketolithocholic acid + NADPH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 30000, SDS-PAGE Collinsella aerofaciens

Synonyms

Synonyms Comment Organism
7beta-HSDH
-
Collinsella aerofaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
complete inactivation within 400 min Collinsella aerofaciens
50
-
complete inactivation within 5 min Collinsella aerofaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.6
-
Dehydrocholic acid pH 8.0, 25°C Collinsella aerofaciens
15.7
-
NADP+ pH 8.0, 25°C Collinsella aerofaciens
15.8
-
7-ketolithocholic acid pH 8.0, 25°C Collinsella aerofaciens
17.2
-
NADPH pH 8.0, 25°C Collinsella aerofaciens
19.6
-
Ursodeoxycholic acid pH 8.0, 25°C Collinsella aerofaciens