Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Collinsella aerofaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
NADPH | pH 8.0, 25°C | Collinsella aerofaciens | |
0.0052 | - |
7-ketolithocholic acid | pH 8.0, 25°C | Collinsella aerofaciens | |
0.0053 | - |
NADP+ | pH 8.0, 25°C | Collinsella aerofaciens | |
0.0062 | - |
Ursodeoxycholic acid | pH 8.0, 25°C | Collinsella aerofaciens | |
0.0092 | - |
Dehydrocholic acid | pH 8.0, 25°C | Collinsella aerofaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, SDS-PAGE | Collinsella aerofaciens |
56100 | - |
gel filtration | Collinsella aerofaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Collinsella aerofaciens | - |
- |
- |
Collinsella aerofaciens DSM 3979 | - |
- |
- |
Storage Stability | Organism |
---|---|
-20°C, 50 mM potassium phosphate, pH 8.0, stable for at least 3 months with several times of freezing and thawing | Collinsella aerofaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-ketolithocholic acid + NADPH + H+ | - |
Collinsella aerofaciens | ursodeoxycholic acid + NADP+ | only product, 90% conversion, 71% yield | r | |
7-ketolithocholic acid + NADPH + H+ | - |
Collinsella aerofaciens DSM 3979 | ursodeoxycholic acid + NADP+ | only product, 90% conversion, 71% yield | r | |
dehydrocholic acid + NADPH + H+ | - |
Collinsella aerofaciens | ? + NADP+ + H+ | - |
? | |
dehydrocholic acid + NADPH + H+ | - |
Collinsella aerofaciens DSM 3979 | ? + NADP+ + H+ | - |
? | |
ursodeoxycholic acid + NADP+ | - |
Collinsella aerofaciens | 7-ketolithocholic acid + NADPH + H+ | - |
r | |
ursodeoxycholic acid + NADP+ | - |
Collinsella aerofaciens DSM 3979 | 7-ketolithocholic acid + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, SDS-PAGE | Collinsella aerofaciens |
Synonyms | Comment | Organism |
---|---|---|
7beta-HSDH | - |
Collinsella aerofaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
complete inactivation within 400 min | Collinsella aerofaciens |
50 | - |
complete inactivation within 5 min | Collinsella aerofaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.6 | - |
Dehydrocholic acid | pH 8.0, 25°C | Collinsella aerofaciens | |
15.7 | - |
NADP+ | pH 8.0, 25°C | Collinsella aerofaciens | |
15.8 | - |
7-ketolithocholic acid | pH 8.0, 25°C | Collinsella aerofaciens | |
17.2 | - |
NADPH | pH 8.0, 25°C | Collinsella aerofaciens | |
19.6 | - |
Ursodeoxycholic acid | pH 8.0, 25°C | Collinsella aerofaciens |