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Literature summary for 1.1.1.202 extracted from

  • Qi, X.; Zhu, J.; Luo, Y.; Lin, J.; Wang, X.; Ju, Z.; Chen, F.; Zhu, X.; Chen, H.; Sun, W.; Wang, L.
    Overexpression and characterization of the gene encoding 1,3-propanediol oxidoreductase of Citrobacter freundii (2014), Key Eng. Mater., 636, 121-124.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene dhaT, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Citrobacter freundii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Citrobacter freundii
8.9
-
3-hydroxypropanal pH and temperature not specified in the publication Citrobacter freundii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
-
Citrobacter freundii
440000
-
recombinant His6-tagged enzyme, gel filtration Citrobacter freundii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxypropanal + NADH + H+ Citrobacter freundii
-
propane-1,3-diol + NAD+
-
r
propane-1,3-diol + NAD+ Citrobacter freundii
-
3-hydroxypropanal + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Citrobacter freundii P45513
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Citrobacter freundii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropanal + NADH + H+
-
Citrobacter freundii propane-1,3-diol + NAD+
-
r
propane-1,3-diol + NAD+
-
Citrobacter freundii 3-hydroxypropanal + NADH + H+
-
r

Subunits

Subunits Comment Organism
homodecamer 10 * 42927, sequence calculation, 10 * 43000, recombinant His6-tagged enzyme, SDS-PAGE Citrobacter freundii

Synonyms

Synonyms Comment Organism
1,3-propanediol oxidoreductase
-
Citrobacter freundii
DhaT
-
Citrobacter freundii
PDOR
-
Citrobacter freundii

Cofactor

Cofactor Comment Organism Structure
additional information PDOR requires NAD(H) as a cofactor, but the highly conserved NAD(H) binding fingerprint pattern G-X-G-X-X-G is not present in the amino acid sequence Citrobacter freundii
NAD+
-
Citrobacter freundii
NADH
-
Citrobacter freundii

General Information

General Information Comment Organism
evolution enzyme PDOR shows high similarity with member of the family of type III alcohol dehydrogenases. PDOR requires NAD(H) as a cofactor, but the highly conserved NAD(H) binding fingerprint pattern G-X-G-X-X-G is not present in the amino acid sequence. This is also characteristic of most type III alcohol dehydrogenases Citrobacter freundii
metabolism 1,3-propanediol oxidoreductase (PDOR) is the rate-limiting enzyme in 1,3-propandiol synthesis biological pathway. 3-Hydroxypropanal is an intermediary metabolite in the 1,3-propanediol synthesis pathway. It is also an inhibitor to the activity of glycerol dehydratase (GDHt) and PDOR. PDOR is the key rate-limiting enzyme of the 3-HPA transformation and 1,3-PD formation when high concentration of glycerol is used in fermentation Citrobacter freundii