Literature summary for 1.1.1.203 extracted from

Yoon, S.H.; Moon, T.S.; Iranpour, P.; Lanza, A.M.; Prather, K.J.
Cloning and characterization of uronate dehydrogenases from two pseudomonads and Agrobacterium tumefaciens strain C58 (2009), J. Bacteriol., 191, 1565-1573.

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Cloned(Commentary)

Cloned (Comment)	Organism
udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC	Pseudomonas syringae pv. tomato
udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC	Agrobacterium tumefaciens
udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC	Pseudomonas putida KT2440

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	D-galacturonate	-	Pseudomonas syringae pv. tomato
0.1	-	D-galacturonate	-	Pseudomonas putida KT2440
0.16	-	D-galacturonate	-	Agrobacterium tumefaciens
0.17	-	NAD+	-	Pseudomonas syringae pv. tomato
0.18	-	NAD+	-	Agrobacterium tumefaciens
0.21	-	NAD+	-	Pseudomonas putida KT2440
0.25	-	D-glucuronate	-	Pseudomonas putida KT2440
0.28	-	D-glucuronate	-	Pseudomonas syringae pv. tomato
0.37	-	D-glucuronate	-	Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	x * 30000, SDS-PAGE	Pseudomonas syringae pv. tomato
30000	-	x * 30000, SDS-PAGE	Pseudomonas putida KT2440
32000	-	x * 32000, SDS-PAGE	Agrobacterium tumefaciens

Organism

Organism	UniProt	Comment	Textmining
Agrobacterium tumefaciens	Q7CRQ0	-	-
Agrobacterium tumefaciens C58 / ATCC 33970	Q7CRQ0	-	-
Pseudomonas putida KT2440	Q88NN6	-	-
Pseudomonas syringae pv. tomato	Q888H1	-	-
Pseudomonas syringae pv. tomato DC3000	Q888H1	-	-

Purification (Commentary)

Purification (Comment)	Organism
gel filtration	Pseudomonas syringae pv. tomato
gel filtration	Agrobacterium tumefaciens
gel filtration	Pseudomonas putida KT2440

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-galacturonate + NAD+ + H2O	-	Pseudomonas syringae pv. tomato	D-galactarate + NADH + H+	-	?
D-galacturonate + NAD+ + H2O	-	Agrobacterium tumefaciens	D-galactarate + NADH + H+	-	?
D-galacturonate + NAD+ + H2O	-	Pseudomonas putida KT2440	D-galactarate + NADH + H+	-	?
D-galacturonate + NAD+ + H2O	-	Pseudomonas syringae pv. tomato DC3000	D-galactarate + NADH + H+	-	?
D-galacturonate + NAD+ + H2O	-	Agrobacterium tumefaciens C58 / ATCC 33970	D-galactarate + NADH + H+	-	?
D-glucuronate + NAD+ + H2O	-	Pseudomonas syringae pv. tomato	D-glucaric acid + NADH + H+	-	?
D-glucuronate + NAD+ + H2O	-	Agrobacterium tumefaciens	D-glucaric acid + NADH + H+	-	?
D-glucuronate + NAD+ + H2O	-	Pseudomonas putida KT2440	D-glucaric acid + NADH + H+	-	?
D-glucuronate + NAD+ + H2O	-	Pseudomonas syringae pv. tomato DC3000	D-glucaric acid + NADH + H+	-	?
D-glucuronate + NAD+ + H2O	-	Agrobacterium tumefaciens C58 / ATCC 33970	D-glucaric acid + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
?	x * 32000, SDS-PAGE	Agrobacterium tumefaciens
?	x * 30000, SDS-PAGE	Pseudomonas syringae pv. tomato
?	x * 30000, SDS-PAGE	Pseudomonas putida KT2440

Synonyms

Synonyms	Comment	Organism
Udh	-	Pseudomonas syringae pv. tomato
Udh	-	Agrobacterium tumefaciens
Udh	-	Pseudomonas putida KT2440

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.24	-	D-galacturonate	-	Pseudomonas syringae pv. tomato
0.3	-	D-galacturonate	-	Pseudomonas putida KT2440
0.55	-	D-glucuronate	-	Pseudomonas putida KT2440
0.74	-	D-glucuronate	-	Pseudomonas syringae pv. tomato
0.92	-	D-galacturonate	-	Agrobacterium tumefaciens
1.9	-	D-glucuronate	-	Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Pseudomonas putida KT2440
8	-	-	Pseudomonas syringae pv. tomato
8	-	-	Agrobacterium tumefaciens

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Pseudomonas syringae pv. tomato
NAD+	-	Agrobacterium tumefaciens
NAD+	-	Pseudomonas putida KT2440

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Release 2023.1 (January 2023)