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Literature summary for 1.1.1.205 extracted from

  • Ishii, K.; Shiio, I.
    Regulation of purine ribonucleotide synthesis by end product inhibition. I. Effect of purine nucleotides on inosine-5-phosphate dehydrogenase, xanthosine-5-phosphate aminase and adenylosuccinate lyase of Bacillus subtilis (1968), J. Biochem., 63, 661-669.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
glutathione removal of glutathione from the reaction mixture causes 35% loss of acitivity Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxy-ATP 18% inhibition at 0.5 mM Bacillus subtilis
2'-deoxy-GDP 12% inhibition at 0.5 mM Bacillus subtilis
2'-deoxy-GTP 21% inhibition at 0.5 mM Bacillus subtilis
ATP at high concentration of IMP and KCl Bacillus subtilis
GMP competitive, 58% inhibition at 0.5 mM Bacillus subtilis
additional information
-
 Bacillus subtilis
xanthosine 5'-phosphate 39% competitive, inhibition at 0.5 mM Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0256
-
 IMP
-
 Bacillus subtilis
0.67
-
 NAD+
-
 Bacillus subtilis
10
-
 K+
-
 Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
 Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 M strain
-
Bacillus subtilis M
-
 M strain
-

Purification (Commentary)

Purification (Comment) Organism
partial purification Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.25
-
-
 Bacillus subtilis

Storage Stability

Storage Stability Organism
frozen, 2 weeks, no loss of activity Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine 5'-phosphate + NAD+ + H2O
-
 Bacillus subtilis xanthosine 5'-phosphate + NADH
-
 ?
inosine 5'-phosphate + NAD+ + H2O
-
 Bacillus subtilis M xanthosine 5'-phosphate + NADH
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2
-
 60% loss of activity within 4 days Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+ can not be replaced by NADP+ Bacillus subtilis