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Literature summary for 1.1.1.205 extracted from

  • Krishnaiah, K.V.
    Inosinic acid 5-monophosphate dehydrogenase of Escherichia coli: purification by affinity chromatography and some properties (1975), Arch. Biochem. Biophys., 170, 567-575.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
GMP weak and competitive inhibition, strong binding, dissociation by 3 M urea Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli
0.013
-
IMP
-
Escherichia coli
0.0132
-
inosine 5'-phosphate
-
Escherichia coli
0.17
-
NAD+
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53770
-
calculated from amino acid composition Escherichia coli
54000
-
SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
inosine 5'-phosphate + NAD+ + H2O Escherichia coli
-
xanthosine 5'-phosphate + NADH
-
ir

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Escherichia coli
homogeneity Escherichia coli
native enzyme about 20fold to homogeneity by ammonium sulfate fractionation, gel filtration, and affinity chromatography Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
by at least 50fold dilution after denaturation with guanidinium hydrochloride, urea, or SDS, overview Escherichia coli
readily renatured following denaturation with urea, guanidine-HCl and SDS Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli
18.75
-
-
Escherichia coli
18.75
-
purified enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine 5'-phosphate + NAD+ + H2O
-
Escherichia coli xanthosine 5'-phosphate + NADH
-
?
inosine 5'-phosphate + NAD+ + H2O
-
Escherichia coli xanthosine 5'-phosphate + NADH
-
ir

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
inosinic acid 5’-monophosphate dehydrogenase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
39
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.6 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Escherichia coli
0.092
-
GMP
-
Escherichia coli