Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GMP | weak and competitive inhibition, strong binding, dissociation by 3 M urea | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
0.013 | - |
IMP | - |
Escherichia coli | |
0.0132 | - |
inosine 5'-phosphate | - |
Escherichia coli | |
0.17 | - |
NAD+ | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53770 | - |
calculated from amino acid composition | Escherichia coli |
54000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | Escherichia coli | - |
xanthosine 5'-phosphate + NADH | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Escherichia coli |
homogeneity | Escherichia coli |
native enzyme about 20fold to homogeneity by ammonium sulfate fractionation, gel filtration, and affinity chromatography | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
by at least 50fold dilution after denaturation with guanidinium hydrochloride, urea, or SDS, overview | Escherichia coli |
readily renatured following denaturation with urea, guanidine-HCl and SDS | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
18.75 | - |
- |
Escherichia coli |
18.75 | - |
purified enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | - |
Escherichia coli | xanthosine 5'-phosphate + NADH | - |
? | |
inosine 5'-phosphate + NAD+ + H2O | - |
Escherichia coli | xanthosine 5'-phosphate + NADH | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
inosinic acid 5-monophosphate dehydrogenase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
39 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.6 | assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Escherichia coli | |
0.092 | - |
GMP | - |
Escherichia coli |