Cloned (Comment) | Organism |
---|---|
expression of isoyzmes IMPDH I and IMPDH II in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-methylthiazole-4-carboxamide adenine dinucleotide | noncompetitive inhibition, less cytotoxic against K562 tumor cells | Homo sapiens | |
3'-methylthiazole-4-carboxamide adenine dinucleotide | noncompetitive inhibition, less cytotoxic against K562 tumor cells | Homo sapiens | |
beta-methylene-TAD | noncompetitive inhibition | Homo sapiens | |
thiazole-4-carboxamide adenine dinucleotide | i.e. TAD, synthesis, noncompetitive inhibition, conformational analysis, and biological activity of inhibitor analogues, overview, TAD binds to the NAD+ binding site with out isozyme specificity, mechanism and molecular modeling using the enzyme crystal structure, overview, cytotoxic against K562 tumor cells | Homo sapiens | |
tiazofurin | noncompetitive inhibition, cytotoxic against K562 tumor cells | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | Homo sapiens | rate-limiting step of the de novo guanine nucleotide biosynthesis | xanthosine 5'-phosphate + NADH | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
type I and type II isozymes | - |
Purification (Comment) | Organism |
---|---|
recombinant isoyzmes IMPDH I and IMPDH II from Escherichia coli strain BL21(DE3) by two steps of ion exchange chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | - |
Homo sapiens | xanthosine 5'-phosphate + NADH | - |
ir | |
inosine 5'-phosphate + NAD+ + H2O | rate-limiting step of the de novo guanine nucleotide biosynthesis | Homo sapiens | xanthosine 5'-phosphate + NADH | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
IMPDH II | - |
Homo sapiens |
inosine 5'-monophosphate dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition patterns, isozymes I and II | Homo sapiens | |
0.00019 | - |
thiazole-4-carboxamide adenine dinucleotide | isozyme IMPDH I | Homo sapiens | |
0.00024 | - |
thiazole-4-carboxamide adenine dinucleotide | isozyme IMPDH II | Homo sapiens | |
0.00052 | - |
3'-methylthiazole-4-carboxamide adenine dinucleotide | isozyme IMPDH I | Homo sapiens | |
0.00066 | - |
3'-methylthiazole-4-carboxamide adenine dinucleotide | isozyme IMPDH II | Homo sapiens | |
0.0011 | - |
2'-methylthiazole-4-carboxamide adenine dinucleotide | isozyme IMPDH I | Homo sapiens | |
0.0014 | - |
2'-methylthiazole-4-carboxamide adenine dinucleotide | isozyme IMPDH II | Homo sapiens |