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Literature summary for 1.1.1.205 extracted from

  • Pimkin, M.; Markham, G.D.
    The CBS subdomain of inosine 5-monophosphate dehydrogenase regulates purine nucleotide turnover (2008), Mol. Microbiol., 68, 342-359.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information construction of guanine-prototrophic strain by deletion of the cystathione beta-synthase domain from the chromosomal gene of inosine 5'-monophosphate dehydrogenase. Mutant strain exhibits a substantially elevated ATP content and a slightly reduced GTP content. Activities of inosine 5'-monophosphate dehydrogenase, adenylosuccinate synthetase and GMP reductase are two- to threefold lower than in wild-type Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine 5'-phosphate + NAD+
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Escherichia coli xanthosine 5'-phosphate + NADH + H+
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?

Synonyms

Synonyms Comment Organism
IMPDH
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Escherichia coli
inosine 5'-monophosphate dehydrogenase
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Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
assay at Escherichia coli

General Information

General Information Comment Organism
malfunction a guanine-prototrophic Escherichia coli strain, MP101, is constructed with the cystathionine beta-synthase subdomain deleted from the chromosomal gene for IMPDH. The ATP content is substantially elevated in mutant strain MP101 whereas the GTP content is slighty reduced. The activities of IMPDH, adenylosuccinate synthetase and GMP reductase are 2 to 3fold lower in MP101 crude extracts compared with wild-type strain. Results of a comparative analysis of the purine nucleotide pools, fluxes and turnover rates in the mutant and wild-type strains, indicate that the cystathionine beta-synthase subdomain of IMPDH plays an important role in the regulation of purine nucleotide metabolism Escherichia coli