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Literature summary for 1.1.1.208 extracted from

  • Davis, E.M.; Ringer, K.L.; McConkey, M.E.; Croteau, R.
    Monoterpene metabolism. Cloning, expression, and characterization of menthone reductases from peppermint (2005), Plant Physiol., 137, 873-881.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.208

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli Mentha x piperita

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0054
-
 NADP+ pH 9.3, 31°C, recombinant enzyme Mentha x piperita
0.01
-
 NADPH pH 9.3, 31°C, recombinant enzyme Mentha x piperita
0.674
-
 (-)-menthone pH 9.3, 31°C, recombinant enzyme Mentha x piperita
1
-
 (+)-isomenthone above, pH 9.3, 31°C, recombinant enzyme Mentha x piperita

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35722
-
 x * 35722, amino acid sequence calculation Mentha x piperita

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-isomenthone + NADPH Mentha x piperita low activity (+)-isomenthol + NADP+
-
 ?
(-)-menthone + NADPH + H+ Mentha x piperita
-
 (3S)-neomenthol + NADP+
-
 r
additional information Mentha x piperita primary monoterpene biosynthesis pathway, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mentha x piperita
-
 cv. Black Mitchum
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme partially Mentha x piperita

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Mentha x piperita
-
oil gland secretory cell
-
 Mentha x piperita
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-isomenthone + NADPH low activity Mentha x piperita (+)-isomenthol + NADP+
-
 ?
(-)-menthone + NADPH + H+
-
 Mentha x piperita (3S)-neomenthol + NADP+
-
 r
(-)-menthone + NADPH + H+ low activity in the reverse reaction Mentha x piperita (3S)-neomenthol + NADP+ (3S)-neomenthol is the main product, product determination by gas chromatography, overview r
additional information primary monoterpene biosynthesis pathway, overview Mentha x piperita ?
-
 ?
additional information monoterpenol is a poor substrate Mentha x piperita ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35722, amino acid sequence calculation Mentha x piperita

Synonyms

Synonyms Comment Organism
(-)-menthone:(3S)-neomenthol reductase
-
 Mentha x piperita
MNR
-
 Mentha x piperita

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
31
-
 assay at Mentha x piperita

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
 NADPH pH 9.3, 31°C, recombinant enzyme Mentha x piperita
0.06
-
 (-)-menthone pH 9.3, 31°C, recombinant enzyme Mentha x piperita

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
-
 Mentha x piperita

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10.5 50% of maximal activity at pH 6.5 and pH 10.5 Mentha x piperita

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Mentha x piperita
NADPH
-
 Mentha x piperita