Protein Variants | Comment | Organism |
---|---|---|
F115Y | stable and active | Homo sapiens |
T113A | stable and active | Homo sapiens |
T113A/F115Y | 10fold decrease in kcat value, does not bind to inhibitor (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | Homo sapiens |
T113V | inhibition constants and vibrational frequencies of inhibitor (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid in the active site | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isoform hALR2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-glyceraldehyde + NADPH | - |
Homo sapiens | glycerol + NADP+ | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | mutant T113A/C303D, pH 7.0, 22°C | Homo sapiens | |
0.00006 | - |
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | mutant T113A/C303NpH 7.0, 22°C | Homo sapiens | |
0.00011 | - |
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | mutant T113A, pH 7.0, 22°C | Homo sapiens | |
0.00029 | - |
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | mutant S302R/C303D, pH 7.0, 22°C | Homo sapiens | |
0.00029 | - |
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | mutant T113V, pH 7.0, 22°C | Homo sapiens | |
0.00051 | - |
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | mutant C303D, pH 7.0, 22°C | Homo sapiens | |
0.00062 | - |
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | mutant F115Y, pH 7.0, 22°C | Homo sapiens | |
0.0008 | - |
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | mutant C303N, pH 7.0, 22°C | Homo sapiens | |
0.002 | - |
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | mutant C80N, pH 7.0, 22°C | Homo sapiens | |
0.002 | - |
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | wild-type, pH 7.0, 22°C | Homo sapiens | |
0.0039 | - |
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | mutant T113Y, pH 7.0, 22°C | Homo sapiens | |
0.004 | - |
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | mutant T113Y/S302R/C303D, pH 7.0, 22°C | Homo sapiens | |
0.0046 | - |
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | mutant C80A, pH 7.0, 22°C | Homo sapiens | |
0.0078 | - |
(5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid | mutant C80N/T113Y/S302R/C303D, pH 7.0, 22°C | Homo sapiens |