Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 100% inhibition at 0.1 mM | Rattus norvegicus | |
iodoacetate | 50% inhibition at 0.1 mM | Rattus norvegicus | |
p-chloromercuribenzoate | non-competitive with respect to dihydrocortisone 46-100% inhibition at 0.01 mM, preincubation with NADH lowers the inhibitory effect | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
5beta-pregnane-17alpha,20beta,21-triol-3,11-dione | - |
Rattus norvegicus | |
0.015 | - |
NADH | - |
Rattus norvegicus | |
0.04 | - |
NAD+ | - |
Rattus norvegicus | |
0.04 | - |
NADP+ | - |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Ca2(PO4)2 gel extraction, ethanol precipitation | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | also present in kidney and testis | Rattus norvegicus | - |
additional information | low activities found in dog and rabbit liver, no significant activities are detected in extracts of beef, hog, guinea pig, mouse and human liver | Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-10°C, 3 weeks, activity declines about 50% | Rattus norvegicus |
-10°C, most purified preparation, 5 days, loses between 80% and 100% of activity | Rattus norvegicus |
3°C, first ammonium sulfate fraction, stable at least 3 weeks | Rattus norvegicus |
3°C, most purified preparation, 5 days, loses 50% of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5beta-pregnane-17alpha,20beta,21-triol-3,11-dione + NAD(P)H | - |
Rattus norvegicus | 5beta-pregnane-3alpha,17alpha,20beta,21-tetraol-11-one + NAD(P)+ | - |
r | |
androstan-17beta-ol-3-one + NADH | - |
Rattus norvegicus | androstan-3alpha,17beta-diol + NAD+ | - |
? | |
androstan-3,17-dione + NADH | - |
Rattus norvegicus | androstan-3alpha-ol-17-one + NAD+ | - |
? | |
etiocholane-3,17-dione + NADH | - |
Rattus norvegicus | etiocholan-3alpha-ol-17-one + NAD+ | - |
? | |
pregnane-11beta,17alpha,21-triol-3,20-dione + NADH | - |
Rattus norvegicus | pregnane-3alpha,11beta,17alpha,21-tetrol-20-one + NAD+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus | |
NADH | - |
Rattus norvegicus | |
NADP+ | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |