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Literature summary for 1.1.1.213 extracted from

  • Rani, K.; Garg, P.; Pundir, C.S.
    Discrete analysis of bile acid in serum and bile with 3alpha-hydroxysteroid dehydrogenase and diaphorase immobilized onto alkylamine glass beads (2006), Indian J. Biochem. Biophys., 43, 98-104.
    View publication on PubMed

Application

Application Comment Organism
diagnostics co-immobilization of the enzyme with diaphorase of Clostridum sp. onto alkylamine glass beads through glutaraldehyde coupling for determination of bile acids in serum and bile in a cost-effective colorimetric assay Comamonas testosteroni

Protein Variants

Protein Variants Comment Organism
additional information co-immobilization of the enzyme with diaphorase of Clostridum sp. onto alkylamine glass beads through glutaraldehyde coupling for determination of bile acids, method opimization and evaluation, loss of 30% of activity after 4 months of regular use, overview Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-

Synonyms

Synonyms Comment Organism
3alpha-hydroxysteroid dehydrogenase
-
Comamonas testosteroni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
bile acid determination assay at Comamonas testosteroni

Cofactor

Cofactor Comment Organism Structure
NADH
-
Comamonas testosteroni