General Stability | Organism |
---|---|
enzyme stable without 2-mercaptoethanol | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5beta-dihydrotestosterone | - |
Oryctolagus cuniculus | |
additional information | 5beta-dihydrotestosterone, lithocholic acid, androsterone and estradiol inhibit competitively toward morphine binding; little inhibitory effect: indomethacin, prostaglandins E2, D2; no or weak inhibition by pyrazole, barbital | Oryctolagus cuniculus | |
NEM | - |
Oryctolagus cuniculus | |
p-chloromercuribenzoic acid | - |
Oryctolagus cuniculus | |
Phenylarsine oxide | - |
Oryctolagus cuniculus | |
quercetin | - |
Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
codeine | with NAD+ | Oryctolagus cuniculus | |
0.12 | - |
1-Indanol | cosubstrate NAD+ | Oryctolagus cuniculus | |
0.39 | - |
Dihydrocodeine | cosubstrate NAD+ | Oryctolagus cuniculus | |
0.54 | - |
cyclohexene-2-ol | cosubstrate NAD+ | Oryctolagus cuniculus | |
0.64 | - |
normorphine | cosubstrate NAD+ | Oryctolagus cuniculus | |
0.72 | - |
morphine | cosubstrate NAD+ | Oryctolagus cuniculus | |
0.92 | - |
3-hydroxyhexobarbital | cosubstrate NAD+ | Oryctolagus cuniculus | |
1.48 | - |
ethylmorphine | cosubstrate NAD+ | Oryctolagus cuniculus | |
1.75 | - |
benzenedihydrodiol | cosubstrate NAD+ | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
microsome | microsomal fraction | Oryctolagus cuniculus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
native enzyme, gel filtration | Oryctolagus cuniculus |
36000 | - |
denatured enzyme, SDS-PAGE | Oryctolagus cuniculus |
38000 | - |
1 * 38000, SDS-PAGE | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
Japanese white rabbit | - |
Purification (Comment) | Organism |
---|---|
gel filtration, various chromatographic techniques | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.43 | - |
morphine, NAD+ | Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-80°C, 20% glycerol, v/v, stable at least four months | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-indanol + NADP+ | - |
Oryctolagus cuniculus | 1-indanone + NADPH | - |
? | |
3-hydroxyhexobarbital + NADP+ | does not serve as a substrate for guinea pig enzyme | Oryctolagus cuniculus | ? | - |
? | |
benzenedihydrodiol + NADP+ | - |
Oryctolagus cuniculus | ? | - |
? | |
codeine + NAD(P)+ | codeine best substrate | Oryctolagus cuniculus | codeinone + NAD(P)H | - |
? | |
cyclohexene-2-ol + NADP+ | - |
Oryctolagus cuniculus | cyclohexene-2-one + NADPH | - |
? | |
dihydrocodeine + NADP+ | - |
Oryctolagus cuniculus | dihydrocodeinone + NADPH | - |
? | |
ethylmorphine + NAD(P)+ | - |
Oryctolagus cuniculus | ? | - |
? | |
morphine + NADP+ | enzyme acitivity with NAD+ four times that with NADP+ | Oryctolagus cuniculus | morphinone + NADPH + H+ | - |
? | |
normorphine + NAD(P)+ | - |
Oryctolagus cuniculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, SDS-PAGE | Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
enzyme assay | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
with NADP+ as cofactor, in glycine-NaCl-NaOH buffer | Oryctolagus cuniculus |
9.4 | - |
with NAD+ as cofactor, in glycine-NaCl-NaOH buffer | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | NAD+ preferred cofactor | Oryctolagus cuniculus |