Cloned (Comment) | Organism |
---|---|
functional expression in tobacco protoplasts via electroporation, subcloning and overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1, the recombinant Escherichia coli strain shows no enzyme activity | Gerbera hybrid cultivar |
overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 | Matthiola incana |
overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 | Dianthus caryophyllus |
overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 | Rosa hybrid cultivar |
overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 | Solanum lycopersicum |
overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 | Callistephus chinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Matthiola incana | enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway | ? | - |
? | |
additional information | Gerbera hybrid cultivar | enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway | ? | - |
? | |
additional information | Dianthus caryophyllus | enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway | ? | - |
? | |
additional information | Rosa hybrid cultivar | enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway | ? | - |
? | |
additional information | Solanum lycopersicum | enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway | ? | - |
? | |
additional information | Callistephus chinensis | enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway | ? | - |
? | |
additional information | Rosa hybrid cultivar Kardinal | enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Callistephus chinensis | P51103 | line 101 | - |
Dianthus caryophyllus | P51104 | Tanga | - |
Gerbera hybrid cultivar | P51105 | v. Terra Regina | - |
Matthiola incana | - |
line 10 | - |
no activity in Escherichia coli | - |
- |
- |
no activity in Nicotiana tabacum | - |
- |
- |
no activity in Saccharomyces cerevisiae | - |
- |
- |
Rosa hybrid cultivar | Q41158 | Kardinal | - |
Rosa hybrid cultivar Kardinal | Q41158 | Kardinal | - |
Solanum lycopersicum | P51107 | line 19 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Matthiola incana | - |
flower | - |
Gerbera hybrid cultivar | - |
flower | - |
Dianthus caryophyllus | - |
flower | - |
Rosa hybrid cultivar | - |
flower | - |
Solanum lycopersicum | - |
flower | - |
Callistephus chinensis | - |
leaf | young | Matthiola incana | - |
leaf | young | Gerbera hybrid cultivar | - |
leaf | young | Dianthus caryophyllus | - |
leaf | young | Rosa hybrid cultivar | - |
leaf | young | Solanum lycopersicum | - |
leaf | young | Callistephus chinensis | - |
petal | - |
Matthiola incana | - |
petal | - |
Gerbera hybrid cultivar | - |
petal | - |
Dianthus caryophyllus | - |
petal | - |
Rosa hybrid cultivar | - |
petal | - |
Solanum lycopersicum | - |
petal | - |
Callistephus chinensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3R)-(+)-dihydrokaempferol + NADPH | - |
Matthiola incana | (2R,3S,4S)-cis-3,4-leucopelargonidin + NADP+ | - |
? | |
(2R,3R)-(+)-dihydrokaempferol + NADPH | - |
Gerbera hybrid cultivar | (2R,3S,4S)-cis-3,4-leucopelargonidin + NADP+ | - |
? | |
(2R,3R)-(+)-dihydrokaempferol + NADPH | - |
Dianthus caryophyllus | (2R,3S,4S)-cis-3,4-leucopelargonidin + NADP+ | - |
? | |
(2R,3R)-(+)-dihydrokaempferol + NADPH | - |
Callistephus chinensis | (2R,3S,4S)-cis-3,4-leucopelargonidin + NADP+ | - |
? | |
(2R,3R)-dihydromyricetin + NADPH | - |
Matthiola incana | (2R,3S,4R)-3,4-leucodelphinidin + NADP+ | - |
? | |
(2R,3R)-dihydromyricetin + NADPH | - |
Gerbera hybrid cultivar | (2R,3S,4R)-3,4-leucodelphinidin + NADP+ | - |
? | |
(2R,3R)-dihydromyricetin + NADPH | - |
Dianthus caryophyllus | (2R,3S,4R)-3,4-leucodelphinidin + NADP+ | - |
? | |
(2R,3R)-dihydromyricetin + NADPH | - |
Rosa hybrid cultivar | (2R,3S,4R)-3,4-leucodelphinidin + NADP+ | - |
? | |
(2R,3R)-dihydromyricetin + NADPH | - |
Solanum lycopersicum | (2R,3S,4R)-3,4-leucodelphinidin + NADP+ | - |
? | |
(2R,3R)-dihydromyricetin + NADPH | - |
Callistephus chinensis | (2R,3S,4R)-3,4-leucodelphinidin + NADP+ | - |
? | |
(2R,3R)-dihydromyricetin + NADPH | - |
Rosa hybrid cultivar Kardinal | (2R,3S,4R)-3,4-leucodelphinidin + NADP+ | - |
? | |
(2R,3R)-dihydroquercetin + NADPH | - |
Matthiola incana | (2R,3S,4R)-leucocyanidin + NADP+ | - |
? | |
(2R,3R)-dihydroquercetin + NADPH | - |
Gerbera hybrid cultivar | (2R,3S,4R)-leucocyanidin + NADP+ | - |
? | |
(2R,3R)-dihydroquercetin + NADPH | - |
Dianthus caryophyllus | (2R,3S,4R)-leucocyanidin + NADP+ | - |
? | |
(2R,3R)-dihydroquercetin + NADPH | - |
Rosa hybrid cultivar | (2R,3S,4R)-leucocyanidin + NADP+ | - |
? | |
(2R,3R)-dihydroquercetin + NADPH | - |
Solanum lycopersicum | (2R,3S,4R)-leucocyanidin + NADP+ | - |
? | |
(2R,3R)-dihydroquercetin + NADPH | - |
Callistephus chinensis | (2R,3S,4R)-leucocyanidin + NADP+ | - |
? | |
(2R,3R)-dihydroquercetin + NADPH | - |
Rosa hybrid cultivar Kardinal | (2R,3S,4R)-leucocyanidin + NADP+ | - |
? | |
additional information | stereospecific reaction | Callistephus chinensis | ? | - |
? | |
additional information | enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway | Matthiola incana | ? | - |
? | |
additional information | enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway | Gerbera hybrid cultivar | ? | - |
? | |
additional information | enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway | Dianthus caryophyllus | ? | - |
? | |
additional information | enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway | Rosa hybrid cultivar | ? | - |
? | |
additional information | enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway | Solanum lycopersicum | ? | - |
? | |
additional information | enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway | Callistephus chinensis | ? | - |
? | |
additional information | no activity with (+)-dihydrokaempferol | Rosa hybrid cultivar | ? | - |
? | |
additional information | no activity with (+)-dihydrokaempferol | Solanum lycopersicum | ? | - |
? | |
additional information | enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway | Rosa hybrid cultivar Kardinal | ? | - |
? | |
additional information | no activity with (+)-dihydrokaempferol | Rosa hybrid cultivar Kardinal | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DFR | - |
Matthiola incana |
DFR | - |
Gerbera hybrid cultivar |
DFR | - |
Dianthus caryophyllus |
DFR | - |
Rosa hybrid cultivar |
DFR | - |
Solanum lycopersicum |
DFR | - |
Callistephus chinensis |
dihydroflavonol 4-reductase | - |
Matthiola incana |
dihydroflavonol 4-reductase | - |
Gerbera hybrid cultivar |
dihydroflavonol 4-reductase | - |
Dianthus caryophyllus |
dihydroflavonol 4-reductase | - |
Rosa hybrid cultivar |
dihydroflavonol 4-reductase | - |
Solanum lycopersicum |
dihydroflavonol 4-reductase | - |
Callistephus chinensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Matthiola incana |
30 | - |
assay at | Gerbera hybrid cultivar |
30 | - |
assay at | Dianthus caryophyllus |
30 | - |
assay at | Rosa hybrid cultivar |
30 | - |
assay at | Solanum lycopersicum |
30 | - |
assay at | Callistephus chinensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Matthiola incana |
7.5 | - |
assay at | Gerbera hybrid cultivar |
7.5 | - |
assay at | Dianthus caryophyllus |
7.5 | - |
assay at | Rosa hybrid cultivar |
7.5 | - |
assay at | Solanum lycopersicum |
7.5 | - |
assay at | Callistephus chinensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Matthiola incana | |
NADPH | - |
Gerbera hybrid cultivar | |
NADPH | - |
Dianthus caryophyllus | |
NADPH | - |
Rosa hybrid cultivar | |
NADPH | - |
Solanum lycopersicum | |
NADPH | - |
Callistephus chinensis |