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Literature summary for 1.1.1.219 extracted from

  • Martens, S.; Teeri, T.; Forkmann, G.
    Heterologous expression of dihydroflavonol 4-reductases from various plants (2002), FEBS Lett., 531, 453-458.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
functional expression in tobacco protoplasts via electroporation, subcloning and overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1, the recombinant Escherichia coli strain shows no enzyme activity Gerbera hybrid cultivar
overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 Matthiola incana
overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 Dianthus caryophyllus
overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 Rosa hybrid cultivar
overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 Solanum lycopersicum
overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 Callistephus chinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Matthiola incana enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway ?
-
?
additional information Gerbera hybrid cultivar enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway ?
-
?
additional information Dianthus caryophyllus enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway ?
-
?
additional information Rosa hybrid cultivar enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway ?
-
?
additional information Solanum lycopersicum enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway ?
-
?
additional information Callistephus chinensis enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway ?
-
?
additional information Rosa hybrid cultivar Kardinal enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway ?
-
?

Organism

Organism UniProt Comment Textmining
Callistephus chinensis P51103 line 101
-
Dianthus caryophyllus P51104 Tanga
-
Gerbera hybrid cultivar P51105 v. Terra Regina
-
Matthiola incana
-
line 10
-
no activity in Escherichia coli
-
-
-
no activity in Nicotiana tabacum
-
-
-
no activity in Saccharomyces cerevisiae
-
-
-
Rosa hybrid cultivar Q41158 Kardinal
-
Rosa hybrid cultivar Kardinal Q41158 Kardinal
-
Solanum lycopersicum P51107 line 19
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Matthiola incana
-
flower
-
Gerbera hybrid cultivar
-
flower
-
Dianthus caryophyllus
-
flower
-
Rosa hybrid cultivar
-
flower
-
Solanum lycopersicum
-
flower
-
Callistephus chinensis
-
leaf young Matthiola incana
-
leaf young Gerbera hybrid cultivar
-
leaf young Dianthus caryophyllus
-
leaf young Rosa hybrid cultivar
-
leaf young Solanum lycopersicum
-
leaf young Callistephus chinensis
-
petal
-
Matthiola incana
-
petal
-
Gerbera hybrid cultivar
-
petal
-
Dianthus caryophyllus
-
petal
-
Rosa hybrid cultivar
-
petal
-
Solanum lycopersicum
-
petal
-
Callistephus chinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3R)-(+)-dihydrokaempferol + NADPH
-
Matthiola incana (2R,3S,4S)-cis-3,4-leucopelargonidin + NADP+
-
?
(2R,3R)-(+)-dihydrokaempferol + NADPH
-
Gerbera hybrid cultivar (2R,3S,4S)-cis-3,4-leucopelargonidin + NADP+
-
?
(2R,3R)-(+)-dihydrokaempferol + NADPH
-
Dianthus caryophyllus (2R,3S,4S)-cis-3,4-leucopelargonidin + NADP+
-
?
(2R,3R)-(+)-dihydrokaempferol + NADPH
-
Callistephus chinensis (2R,3S,4S)-cis-3,4-leucopelargonidin + NADP+
-
?
(2R,3R)-dihydromyricetin + NADPH
-
Matthiola incana (2R,3S,4R)-3,4-leucodelphinidin + NADP+
-
?
(2R,3R)-dihydromyricetin + NADPH
-
Gerbera hybrid cultivar (2R,3S,4R)-3,4-leucodelphinidin + NADP+
-
?
(2R,3R)-dihydromyricetin + NADPH
-
Dianthus caryophyllus (2R,3S,4R)-3,4-leucodelphinidin + NADP+
-
?
(2R,3R)-dihydromyricetin + NADPH
-
Rosa hybrid cultivar (2R,3S,4R)-3,4-leucodelphinidin + NADP+
-
?
(2R,3R)-dihydromyricetin + NADPH
-
Solanum lycopersicum (2R,3S,4R)-3,4-leucodelphinidin + NADP+
-
?
(2R,3R)-dihydromyricetin + NADPH
-
Callistephus chinensis (2R,3S,4R)-3,4-leucodelphinidin + NADP+
-
?
(2R,3R)-dihydromyricetin + NADPH
-
Rosa hybrid cultivar Kardinal (2R,3S,4R)-3,4-leucodelphinidin + NADP+
-
?
(2R,3R)-dihydroquercetin + NADPH
-
Matthiola incana (2R,3S,4R)-leucocyanidin + NADP+
-
?
(2R,3R)-dihydroquercetin + NADPH
-
Gerbera hybrid cultivar (2R,3S,4R)-leucocyanidin + NADP+
-
?
(2R,3R)-dihydroquercetin + NADPH
-
Dianthus caryophyllus (2R,3S,4R)-leucocyanidin + NADP+
-
?
(2R,3R)-dihydroquercetin + NADPH
-
Rosa hybrid cultivar (2R,3S,4R)-leucocyanidin + NADP+
-
?
(2R,3R)-dihydroquercetin + NADPH
-
Solanum lycopersicum (2R,3S,4R)-leucocyanidin + NADP+
-
?
(2R,3R)-dihydroquercetin + NADPH
-
Callistephus chinensis (2R,3S,4R)-leucocyanidin + NADP+
-
?
(2R,3R)-dihydroquercetin + NADPH
-
Rosa hybrid cultivar Kardinal (2R,3S,4R)-leucocyanidin + NADP+
-
?
additional information stereospecific reaction Callistephus chinensis ?
-
?
additional information enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway Matthiola incana ?
-
?
additional information enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway Gerbera hybrid cultivar ?
-
?
additional information enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway Dianthus caryophyllus ?
-
?
additional information enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway Rosa hybrid cultivar ?
-
?
additional information enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway Solanum lycopersicum ?
-
?
additional information enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway Callistephus chinensis ?
-
?
additional information no activity with (+)-dihydrokaempferol Rosa hybrid cultivar ?
-
?
additional information no activity with (+)-dihydrokaempferol Solanum lycopersicum ?
-
?
additional information enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway Rosa hybrid cultivar Kardinal ?
-
?
additional information no activity with (+)-dihydrokaempferol Rosa hybrid cultivar Kardinal ?
-
?

Synonyms

Synonyms Comment Organism
DFR
-
Matthiola incana
DFR
-
Gerbera hybrid cultivar
DFR
-
Dianthus caryophyllus
DFR
-
Rosa hybrid cultivar
DFR
-
Solanum lycopersicum
DFR
-
Callistephus chinensis
dihydroflavonol 4-reductase
-
Matthiola incana
dihydroflavonol 4-reductase
-
Gerbera hybrid cultivar
dihydroflavonol 4-reductase
-
Dianthus caryophyllus
dihydroflavonol 4-reductase
-
Rosa hybrid cultivar
dihydroflavonol 4-reductase
-
Solanum lycopersicum
dihydroflavonol 4-reductase
-
Callistephus chinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Matthiola incana
30
-
assay at Gerbera hybrid cultivar
30
-
assay at Dianthus caryophyllus
30
-
assay at Rosa hybrid cultivar
30
-
assay at Solanum lycopersicum
30
-
assay at Callistephus chinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Matthiola incana
7.5
-
assay at Gerbera hybrid cultivar
7.5
-
assay at Dianthus caryophyllus
7.5
-
assay at Rosa hybrid cultivar
7.5
-
assay at Solanum lycopersicum
7.5
-
assay at Callistephus chinensis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Matthiola incana
NADPH
-
Gerbera hybrid cultivar
NADPH
-
Dianthus caryophyllus
NADPH
-
Rosa hybrid cultivar
NADPH
-
Solanum lycopersicum
NADPH
-
Callistephus chinensis