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Literature summary for 1.1.1.219 extracted from

  • Miyagawa, N.; Miyahara, T.; Okamoto, M.; Hirose, Y.; Sakaguchi, K.; Hatano, S.; Ozeki, Y.
    Dihydroflavonol 4-reductase activity is associated with the intensity of flower colors in Delphinium (2015), Plant Biotechnol., 32, 249-255.
No PubMed abstract available
Search for more literature for 1.1.1.219

Cloned(Commentary)

Cloned (Comment) Organism
gene DcDFR, genotyping, quantitative enzyme expression analysis Delphinium cardinale
gene DgDFR, genotyping, quantitative enzyme expression analysis Delphinium grandiflorum
gene DnDFR, genotyping, quantitative enzyme expression analysis Delphinium nudicaule

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 dihydromyricetin pH 7.0, 30°C Delphinium grandiflorum
0.002
-
 dihydroquercetin pH 7.0, 30°C Delphinium grandiflorum
0.0061
-
 dihydrokaempferol pH 7.0, 30°C Delphinium grandiflorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydrokaempferol + NADPH + H+ Delphinium grandiflorum
-
 leucopelargonidin + NADP+
-
 ?
dihydrokaempferol + NADPH + H+ Delphinium nudicaule
-
 leucopelargonidin + NADP+
-
 ?
dihydrokaempferol + NADPH + H+ Delphinium cardinale
-
 leucopelargonidin + NADP+
-
 ?
dihydromyricetin + NADPH + H+ Delphinium grandiflorum
-
 leucodelphinidin + NADP+
-
 ?
dihydromyricetin + NADPH + H+ Delphinium nudicaule
-
 leucodelphinidin + NADP+
-
 ?
dihydromyricetin + NADPH + H+ Delphinium cardinale
-
 leucodelphinidin + NADP+
-
 ?
dihydroquercetin + NADPH + H+ Delphinium grandiflorum
-
 cis-3,4-leucocyanidin + NADP+
-
 ?
dihydroquercetin + NADPH + H+ Delphinium nudicaule
-
 cis-3,4-leucocyanidin + NADP+
-
 ?
dihydroquercetin + NADPH + H+ Delphinium cardinale
-
 cis-3,4-leucocyanidin + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Delphinium cardinale A0A0C6ENR6 several cultivars
-
Delphinium grandiflorum A0A0C6EUC4 var. chinense, several cultivars
-
Delphinium nudicaule A0A0C6E754 several cultivars
-

Source Tissue

Source Tissue Comment Organism Textmining
flower quantitative enzyme expression analysis in flowers from different cultivars, overview Delphinium grandiflorum
-
flower quantitative enzyme expression analysis in flowers from different cultivars, overview Delphinium nudicaule
-
flower quantitative enzyme expression analysis in flowers from different cultivars, overview Delphinium cardinale
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrokaempferol + NADPH + H+
-
 Delphinium grandiflorum leucopelargonidin + NADP+
-
 ?
dihydrokaempferol + NADPH + H+
-
 Delphinium nudicaule leucopelargonidin + NADP+
-
 ?
dihydrokaempferol + NADPH + H+
-
 Delphinium cardinale leucopelargonidin + NADP+
-
 ?
dihydrokaempferol + NADPH + H+ best substrate Delphinium grandiflorum leucopelargonidin + NADP+
-
 ?
dihydrokaempferol + NADPH + H+ best substrate Delphinium nudicaule leucopelargonidin + NADP+
-
 ?
dihydromyricetin + NADPH + H+
-
 Delphinium grandiflorum leucodelphinidin + NADP+
-
 ?
dihydromyricetin + NADPH + H+
-
 Delphinium nudicaule leucodelphinidin + NADP+
-
 ?
dihydromyricetin + NADPH + H+
-
 Delphinium cardinale leucodelphinidin + NADP+
-
 ?
dihydromyricetin + NADPH + H+ 14% of the activity with dihydrokaempferol Delphinium grandiflorum leucodelphinidin + NADP+
-
 ?
dihydromyricetin + NADPH + H+ 23% of the activity with dihydrokaempferol Delphinium nudicaule leucodelphinidin + NADP+
-
 ?
dihydroquercetin + NADPH + H+
-
 Delphinium grandiflorum cis-3,4-leucocyanidin + NADP+
-
 ?
dihydroquercetin + NADPH + H+
-
 Delphinium nudicaule cis-3,4-leucocyanidin + NADP+
-
 ?
dihydroquercetin + NADPH + H+
-
 Delphinium cardinale cis-3,4-leucocyanidin + NADP+
-
 ?
dihydroquercetin + NADPH + H+ 11% of the activity with dihydrokaempferol Delphinium nudicaule cis-3,4-leucocyanidin + NADP+
-
 ?
dihydroquercetin + NADPH + H+ 14% of the activity with dihydrokaempferol Delphinium grandiflorum cis-3,4-leucocyanidin + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
DcDFR
-
 Delphinium cardinale
DFR
-
 Delphinium grandiflorum
DFR
-
 Delphinium nudicaule
DFR
-
 Delphinium cardinale
DgDFR
-
 Delphinium grandiflorum
DnDFR
-
 Delphinium nudicaule

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Delphinium grandiflorum
30
-
 assay at Delphinium nudicaule
30
-
 assay at Delphinium cardinale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Delphinium grandiflorum
7
-
 assay at Delphinium nudicaule
7
-
 assay at Delphinium cardinale

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Delphinium grandiflorum
NADPH
-
 Delphinium nudicaule
NADPH
-
 Delphinium cardinale

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0016
-
 dihydromyricetin pH 7.0, 30°C Delphinium cardinale
0.0023
-
 dihydroquercetin pH 7.0, 30°C Delphinium cardinale
0.006
-
 dihydrokaempferol pH 7.0, 30°C Delphinium cardinale
0.0061
-
 dihydrokaempferol pH 7.0, 30°C Delphinium nudicaule