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Literature summary for 1.1.1.22 extracted from
- Recombinant UDP-glucose dehydrogenase from soybean (2002), Plant Physiol. Biochem., 40, 1011-1017.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no substrate inhibition, K+ is not inhibitory up to 500 mM, not inhibitory: dimethyldicarbonate, EDTA | Glycine max | |
| NADH | 0.1 mM, presence of 0.1 mM NAD+, 44% inhibition, presence of 0.5 mM NAD+, 6% inhibition | Glycine max |  |
| p-chloromercuribenzoate | 0.001 mM, 66% inhibition | Glycine max | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
UDP-glucose | pH 8.7, 25°C | Glycine max | |
| 0.07 | - |
NAD+ | pH 8.7, 25°C | Glycine max | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
gel filtration | Glycine max |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
recombinant enzyme | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no substrate: UDP-galactose, UDP-N-galactosamin, ADP-glucose, GDP-glucose, GDP-mannose | Glycine max | ? | - |
? | |
| UDP-glucose + 2 NAD+ + H2O | NADP+, about 1% of activity of NAD+ | Glycine max | UDP-glucuronate + 2 NADH + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 55000, SDS-PAGE | Glycine max |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Glycine max |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
rapid and irreversible inactivation above | Glycine max |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
- |
Glycine max |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Glycine max | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
p-chloromercuribenzoate | pH 8.7, 25°C | Glycine max | |
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