Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant lacking PA2022 activity and double mutant lacking PA2022 and isoform PA3559 activity are more susceptible to chloramphenicol, ceffotaxime, and ampicillin | Pseudomonas aeruginosa |
additional information | mutant lacking PA3559 activity shows reduced resistance to polymyxin B | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 84% residual activity at 1 mM | Pseudomonas aeruginosa | |
EDTA | 83% residual activity at 1 mM | Pseudomonas aeruginosa | |
Mn2+ | 84% residual activity at 1 mM | Pseudomonas aeruginosa | |
NADH | product inhibition, 26% residual activity at 0.05 mM; product inhibition, 70% residual activity at 0.05 mM | Pseudomonas aeruginosa | |
UDP-glucuronic acid | product inhibition, 44% residual activity at 1 mM; product inhibition, 79% residual activity at 1 mM | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
UDP-glucose | pH 7.5, 22°C | Pseudomonas aeruginosa | |
0.4 | - |
UDP-glucose | pH 7.5, 22°C | Pseudomonas aeruginosa | |
0.47 | - |
NAD+ | pH 7.5, 22°C | Pseudomonas aeruginosa | |
1.99 | - |
NAD+ | pH 7.5, 22°C | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54500 | - |
54500, calculated | Pseudomonas aeruginosa |
57400 | - |
57400, calculated | Pseudomonas aeruginosa |
104000 | - |
gel filtration | Pseudomonas aeruginosa |
117000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | O86422 | isoform PA2022, constitutively expressed | - |
Pseudomonas aeruginosa | Q9HY58 | isoform PA3559, expressed primarily in low concentrations of Mg2+ | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
TDP-glucose + NAD+ + H2O | 38.5% of the rate with UDP-glucose | Pseudomonas aeruginosa | TDP-glucuronate + NADH | - |
? | |
UDP-galactose + NAD+ + H2O | 11.9% of the rate with UDP-glucose | Pseudomonas aeruginosa | UDP-galacturonate + NADH | - |
? | |
UDP-galactose + NAD+ + H2O | 6.4% of the rate with UDP-glucose | Pseudomonas aeruginosa | UDP-galacturonate + NADH | - |
? | |
UDP-glucose + 2 NAD+ + H2O | - |
Pseudomonas aeruginosa | UDP-glucuronate + 2 NADH + 2 H+ | - |
? | |
UDP-N-acetylglucosamine + NAD+ + H2O | 35% of the rate with UDP-glucose | Pseudomonas aeruginosa | ? + NADH | - |
? | |
UDP-N-acetylglucosamine + NAD+ + H2O | 6.3% of the rate with UDP-glucose | Pseudomonas aeruginosa | ? + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 54500, calculated | Pseudomonas aeruginosa |
dimer | 57400, calculated | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
PA2022 | - |
Pseudomonas aeruginosa |
PA3559 | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
UDP-glucose | pH 7.5, 22°C | Pseudomonas aeruginosa | |
0.37 | - |
NAD+ | pH 7.5, 22°C | Pseudomonas aeruginosa | |
0.81 | - |
UDP-glucose | pH 7.5, 22°C | Pseudomonas aeruginosa | |
2.35 | - |
NAD+ | pH 7.5, 22°C | Pseudomonas aeruginosa |