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Literature summary for 1.1.1.22 extracted from

  • Zhou, T.; Song, Y.; Feng, M.; Tan, R.
    Cloning, expression and biochemical characterization of UDP-glucose 6-dehydrogenase, a key enzyme in the biosynthesis of an anti-tumor polysaccharide from the marine fungus Phoma herbarum YS4108 (2011), Process Biochem., 46, 2263-2268.
No PubMed abstract available
Search for more literature for 1.1.1.22

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Phoma herbarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0958
-
 UDP-alpha-D-glucose pH 8.4, 30°C Phoma herbarum
0.168
-
 NAD+ pH 8.4, 30°C Phoma herbarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56300
-
 x * 56300, calculated, x * 60000, SDS-PAGE Phoma herbarum
60000
-
 x * 56300, calculated, x * 60000, SDS-PAGE Phoma herbarum

Organism

Organism UniProt Comment Textmining
Phoma herbarum B5L017
-
-
Phoma herbarum YS4108 B5L017
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + 2 NAD+ + H2O
-
 Phoma herbarum UDP-alpha-D-glucuronate + 2 NADH + 2 H+
-
 ?
UDP-alpha-D-glucose + 2 NAD+ + H2O
-
 Phoma herbarum YS4108 UDP-alpha-D-glucuronate + 2 NADH + 2 H+
-
 ?

Subunits

Subunits Comment Organism
? x * 56300, calculated, x * 60000, SDS-PAGE Phoma herbarum

Synonyms

Synonyms Comment Organism
UDP-GlcDHase
-
 Phoma herbarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Phoma herbarum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50
-
 no enzymic activity above Phoma herbarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
 Phoma herbarum

pH Range

pH Minimum pH Maximum Comment Organism
5.5 11 no enzymic activity beyond Phoma herbarum