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Literature summary for 1.1.1.224 extracted from
- Purification and characterization of mannose 6-phosphate reductase, a potential target for the control of Striga hermonthica and Orobanche ramosa (1999), Aust. J. Plant Physiol., 26, 233-237.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0102 | - |
NADPH | enzyme purified from leaves | Striga hermonthica |  |
| 0.0698 | - |
NADPH | enzyme purified from flowering spikes | Phelipanche ramosa | |
| 5.9 | - |
D-mannose 6-phosphate | enzyme purified from leaves | Striga hermonthica | |
| 8.6 | - |
D-mannose 6-phosphate | enzyme purified from flowering spikes | Phelipanche ramosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
gel filtration with Sephacryl S-200 | Phelipanche ramosa |
| 33000 | - |
gel filtration with Sephacryl S-200 | Striga hermonthica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phelipanche ramosa | - |
broomrape | - |
| Striga hermonthica | - |
witchweed | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Striga hermonthica |
- |
Phelipanche ramosa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Striga hermonthica | - |
| spike | flowering | Phelipanche ramosa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.008 | - |
crude extract from mature leaves | Striga hermonthica |
| 0.014 | - |
crude extract from flowering spikes | Phelipanche ramosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannose 6-phosphate + NADPH | - |
Striga hermonthica | D-mannitol 1-phosphate + NADP+ | - |
? | |
| D-mannose 6-phosphate + NADPH | - |
Phelipanche ramosa | D-mannitol 1-phosphate + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1* 39000, SDS-PAGE | Striga hermonthica |
| monomer | 1* 39000, SDS-PAGE | Phelipanche ramosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Striga hermonthica |
| 45 | - |
- |
Phelipanche ramosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | 75% of maximal activity | Striga hermonthica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Striga hermonthica |
| 7 | - |
- |
Phelipanche ramosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
rapid decrease in the range of basic pH values | Striga hermonthica |
| additional information | - |
rapid decrease in the range of basic pH values | Phelipanche ramosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | 5% activity compared to NADPH | Phelipanche ramosa | |
| NADH | 10% of activity compared to NADPH | Striga hermonthica | |
| NADPH | - |
Striga hermonthica | |
| NADPH | - |
Phelipanche ramosa | |
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