Cloned (Comment) | Organism |
---|---|
- |
Datura stramonium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
NADPH | - |
Datura stramonium | |
0.06 | - |
tropinone | pH 7.5 | Datura stramonium | |
0.063 | - |
tropinone | pH 7.9 | Datura stramonium | |
0.07 | - |
tropinone | pH 6.7 | Datura stramonium | |
0.1 | - |
tropinone | pH 7.0 | Datura stramonium | |
0.11 | - |
tropinone | pH 6.4 | Datura stramonium | |
0.33 | - |
tropinone | pH 5.95 | Datura stramonium | |
1.25 | - |
tropinone | pH 5.4 | Datura stramonium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
3 * 27500 | Atropa belladonna |
27700 | - |
3 * 27700, SDS-PAGE | Datura stramonium |
29000 | - |
3 * 29000 | Datura stramonium |
77700 | - |
gel filtration | Datura stramonium |
78500 | - |
- |
Atropa belladonna |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tropinone + NADPH + H+ | Atropa belladonna | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Datura stramonium | pseudotropine oxidation never observed | pseudotropine + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Atropa belladonna | - |
- |
- |
Datura stramonium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Datura stramonium |
- |
Atropa belladonna |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Atropa belladonna | - |
root | hairy roots | Datura stramonium | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.16 | - |
- |
Datura stramonium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-ethyl-cyclohexanone + NADPH | - |
Datura stramonium | 4-ethylcyclohexanol + NADP+ | - |
? | |
4-methyl-cyclohexanone + NADPH | - |
Datura stramonium | 4-methylcyclohexanol + NADP+ | - |
? | |
6-hydroxytropinone + NADPH | - |
Datura stramonium | ? + NADP+ | - |
? | |
N-methyl-4-piperidone + NADPH | - |
Datura stramonium | N-methylpiperidin-4-ol + NADP+ | - |
? | |
N-propyl-4-piperidone + NADPH | - |
Datura stramonium | N-propylpiperidin-4-ol + NADP+ | - |
? | |
tetrahydrothiopyran-4-one + NADPH | - |
Datura stramonium | tetrahydrothiopyran-4-ol + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Atropa belladonna | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | pseudotropine oxidation never observed | Datura stramonium | pseudotropine + NADP+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 27500 | Atropa belladonna |
trimer | 3 * 29000 | Datura stramonium |
trimer | 3 * 27700, SDS-PAGE | Datura stramonium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | optimum for tetrahydrothiopyran-4-one reduction | Datura stramonium |
6.25 | - |
- |
Datura stramonium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | keeps 90% of maximal activity in the range pH 5.2-8.2 | Datura stramonium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Datura stramonium |