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Literature summary for 1.1.1.236 extracted from

  • Nakajima, K.; Yamashita, A.; Akama, H.; Nakatsu, T.; Kato, H.; Hashimoto, T.; Oda, J.; Yamada, Y.
    Crystal structures of two tropinone reductases: Different reaction stereospecificities in the same protein fold (1998), Proc. Natl. Acad. Sci. USA, 95, 4876-4881.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
TRII cDNA subcloned from pTR2EN induced in Escherichia coli BL21(DE3) Datura stramonium

Crystallization (Commentary)

Crystallization (Comment) Organism
TrII is crystallized by hanging-drop vapor diffusion with 2-methyl-2,4-pentanediol as precipitating reagent, crystals grown by macroseeding technique, crystals belong to the P4.2.2.1.2 space group with cell dimensions of a = b = 62.8 A and c = 128.4 A Datura stramonium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tropinone + NADPH + H+ Datura stramonium
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Hyoscyamus niger
-
pseudotropine + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Datura stramonium
-
-
-
Hyoscyamus niger
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tropinone + NADPH + H+
-
Datura stramonium pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Hyoscyamus niger pseudotropine + NADP+
-
?

Subunits

Subunits Comment Organism
dimer
-
Datura stramonium
dimer
-
Hyoscyamus niger