KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
androst-4-ene-3,17-dione | - |
Alcaligenes sp. | |
0.0036 | - |
testosterone | - |
Alcaligenes sp. | |
0.0064 | - |
17beta-estradiol | - |
Alcaligenes sp. | |
0.009 | - |
testosterone | cofactor S-NAD+ | Alcaligenes sp. | |
0.0139 | - |
17beta-estradiol | cofactor S-NAD+ | Alcaligenes sp. | |
0.027 | - |
NAD+ | - |
Alcaligenes sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Alcaligenes sp. |
68000 | - |
gel filtration | Alcaligenes sp. |
86000 | - |
native PAGE | Alcaligenes sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
testosterone + NAD+ | Alcaligenes sp. | - |
androst-4-ene-3,17-dione + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE cellulose, phenyl-Sepharose, Red-agarose | Alcaligenes sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
335 | - |
- |
Alcaligenes sp. |
Storage Stability | Organism |
---|---|
-20°C, 20 mM potassium phosphate, 50% glycerol, 1 mM dithiotreitol, 1 mM EDTA, pH 7.5, 1 year, no loss in activity | Alcaligenes sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11alpha-hydroxytestosterone + NAD+ | 156% velocity compared to testosterone | Alcaligenes sp. | 11alpha-hydroxyandrost-4-ene-3,17-dione + NADH | - |
r | |
11beta-hydroxytestosterone + NAD+ | 136% velocity compared to testosterone | Alcaligenes sp. | 11beta-hydroxyandrost-4-ene-3,17-dione + NADH | - |
r | |
15alpha-hydroxytestosterone + NAD+ | 131% velocity compared to testosterone | Alcaligenes sp. | 15alpha-hydroxyandrost-4-ene-3,17-dione + NADH | - |
r | |
17beta-hydroxy-5beta-androstan-3-one + NAD+ | 86% velocity compared to testosterone | Alcaligenes sp. | 5beta-androstane-3,17-dione + NADH | - |
r | |
5alpha-androstane-3alpha,17beta-diol + NAD+ | 72% velocity compared to testosterone | Alcaligenes sp. | 5alpha-androstane-3alpha-ol-17-one + NADH | - |
r | |
5alpha-androstane-3beta,17beta-diol + NAD+ | 72% velocity compared to testosterone | Alcaligenes sp. | 5alpha-androstane-3beta-ol-17-one + NADH | - |
r | |
5alpha-dihydrotestosterone + NAD+ | 133% velocity compared to testosterone | Alcaligenes sp. | 5alpha-androstane-3,17-dione + NADH + H+ | - |
r | |
5beta-androstane-3alpha,17beta-diol + NAD+ | 57% velocity compared to testosterone | Alcaligenes sp. | 5beta-androstane-3alpha-ol-17-one + NADH | - |
r | |
6beta-hydroxytestosterone + NAD+ | 84% velocity compared to testosterone | Alcaligenes sp. | 6beta-hydroxyandrost-4-ene-3,17-dione + NADH | - |
r | |
androst-4-ene-3beta,17beta-diol + NAD+ | 75% velocity compared to testosterone | Alcaligenes sp. | androst-4-ene-3beta-ol-17-one + NADH | - |
r | |
androst-5-ene-3beta,17beta-diol + NAD+ | 33% velocity compared to testosterone | Alcaligenes sp. | androst-5-ene-3beta-ol-17-one + NADH | - |
r | |
estradiol + NAD+ | 72% velocity compared to testosterone oxidation | Alcaligenes sp. | estrone + NADH | - |
r | |
testosterone + NAD+ | - |
Alcaligenes sp. | androst-4-ene-3,17-dione + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | Alcaligenes sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
20% activity lost after 1 h in the presence of 52.5% glycerol, half-time for heat inactivation 120 min, activity is rapidly lost above 50°C | Alcaligenes sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of androst-4-ene-3,17-dione | Alcaligenes sp. |
9 | 10 | oxidation of testosterone, equal velocity of oxidation and reduction at pH 8.0 | Alcaligenes sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Alcaligenes sp. |