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Literature summary for 1.1.1.239 extracted from

  • Blomquist, C.H.; Kotts, C.E.; Hakanson, E.Y.
    Microsomal 17beta-hydroxysteroid dehydrogenase of guinea pig liver: detergent solubilization and a comparison of kinetic and stability properties of bound and solubilized forms (1977), J. Steroid Biochem., 8, 193-198.
    View publication on PubMed

General Stability

General Stability Organism
stable at 4°C for at least 10-12 days in the presence of 10 mM 2-mercaptoethanol, activity increases 2fold in the first 2-3 days Cavia porcellus

Inhibitors

Inhibitors Comment Organism Structure
Triton X-100 immediate loss of 60-70% activity, remaining activity is stable for 4 days, competitive vs. testosterone, non-competitive vs. NAD+ Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
testosterone membrane bound Cavia porcellus
0.0073
-
testosterone solubilized with Triton X-100 Cavia porcellus
0.1
-
NAD+ solubilized with Triton X-100 Cavia porcellus
0.164
-
NAD+ membrane bound Cavia porcellus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Cavia porcellus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
176000
-
agarose gel chromatography and density gradient centrifugation of Triton X-100 solubilized microsomes Cavia porcellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
testosterone + NAD+ Cavia porcellus
-
androst-4-ene-3,17-dione + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Cavia porcellus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
testosterone + NAD+
-
Cavia porcellus androst-4-ene-3,17-dione + NADH + H+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
solubilized and membrane bound enzyme Cavia porcellus

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 sharp decrease above pH 10.0 Cavia porcellus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Cavia porcellus