General Stability | Organism |
---|---|
stable at 4°C for at least 10-12 days in the presence of 10 mM 2-mercaptoethanol, activity increases 2fold in the first 2-3 days | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | immediate loss of 60-70% activity, remaining activity is stable for 4 days, competitive vs. testosterone, non-competitive vs. NAD+ | Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
testosterone | membrane bound | Cavia porcellus | |
0.0073 | - |
testosterone | solubilized with Triton X-100 | Cavia porcellus | |
0.1 | - |
NAD+ | solubilized with Triton X-100 | Cavia porcellus | |
0.164 | - |
NAD+ | membrane bound | Cavia porcellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Cavia porcellus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
176000 | - |
agarose gel chromatography and density gradient centrifugation of Triton X-100 solubilized microsomes | Cavia porcellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
testosterone + NAD+ | Cavia porcellus | - |
androst-4-ene-3,17-dione + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Cavia porcellus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
testosterone + NAD+ | - |
Cavia porcellus | androst-4-ene-3,17-dione + NADH + H+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
solubilized and membrane bound enzyme | Cavia porcellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | sharp decrease above pH 10.0 | Cavia porcellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cavia porcellus |