Inhibitors | Comment | Organism | Structure |
---|---|---|---|
17alpha-methyltestosterone | competitive inhibition | Streptomyces exfoliatus | |
androstendione | - |
Streptomyces exfoliatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
testosterone | - |
Streptomyces exfoliatus | |
0.021 | - |
17beta-estradiol | - |
Streptomyces exfoliatus | |
0.125 | - |
NAD+ | - |
Streptomyces exfoliatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
partly purified enzyme, gel filtration | Streptomyces exfoliatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
testosterone + NAD+ | Streptomyces exfoliatus | - |
androst-4-ene-3,17-dione + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces exfoliatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephadex G-200, DEAE-cellulose, 9fold purification | Streptomyces exfoliatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-hydroxy-1alpha-methyl-5alpha-androstan-3-one + NAD+ | 192% velocity compared to testosterone | Streptomyces exfoliatus | 1alpha-methyl-5alpha-androstane-3,17-dione + NADH | - |
r | |
3beta-hydroxy-5alpha-androstane-17-one + NAD+ | 75% velocity compared to testosterone | Streptomyces exfoliatus | 5alpha-androstane-3,17-dione + NADH | - |
r | |
5alpha-androstane-3alpha,17beta-diol + NAD+ | 204% velocity compared to testosterone | Streptomyces exfoliatus | 5alpha-androstane-3alpha-ol-17-one + NADH | - |
r | |
5alpha-androstane-3beta,17beta-diol + NAD+ | 17% velocity compared to testosterone | Streptomyces exfoliatus | 5alpha-androstane-3beta-ol-17-one + NADH | - |
r | |
5alpha-dihydrotestosterone + NAD+ | 119% initial velocity compared to testosterone | Streptomyces exfoliatus | androstanedione + NADH + H+ | - |
r | |
5beta-dihydrotestosterone + NAD+ | 50% initial velocity compared to testosterone | Streptomyces exfoliatus | androstanedione + NADH + H+ | - |
r | |
estradiol + NAD+ | 15% velocity compared to testosterone oxidation | Streptomyces exfoliatus | estrone + NADH | - |
r | |
testosterone + NAD+ | - |
Streptomyces exfoliatus | androst-4-ene-3,17-dione + NADH + H+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Streptomyces exfoliatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 9.5 | - |
Streptomyces exfoliatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Streptomyces exfoliatus |