Application | Comment | Organism |
---|---|---|
medicine | establishment of MCF-7 cells that stably express AKR1C3 to model its over-expression in breast cancer. AKR1C3 expression increases steroid conversion by MCF-7 cells, leading to a pro-estrogenic state. Estrone is reduced fastest by MCF-7 cells expressing AKR1C3 when compared to other substrates at 0.1M. MCF-7 cells expressing AKR1C3 cells proliferate three times faster than parental cells in response to estrone and 17-estradiol. MCF-7 cells expressing AKR1C3 cells also reduce PGD2, limiting its dehydration to form PGJ2 products | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in MCF-7 cell | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
estrone | pH 7.0, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MCF-7 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstenedione + NADPH + H+ | - |
Homo sapiens | testosterone + NADP+ | - |
? | |
estrone + NADPH + H+ | - |
Homo sapiens | 17beta-estradiol + NADP+ | - |
? | |
progesterone + NADPH + H+ | - |
Homo sapiens | 20alpha-hydroxyprogesterone + NADP+ | - |
? | |
prostaglandin D2 + NADPH + H+ | - |
Homo sapiens | 9alpha,11beta-prostaglandin F2 + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
estrone | pH 7.0, 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.127 | - |
estrone | pH 7.0, 37°C | Homo sapiens |