General Stability | Organism |
---|---|
stabilized against heating by 3 mM NAD+ | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaN3 | weak | Pseudomonas sp. | |
SDS | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
NAD+ | with N-acetyl-D-glucosamine as substrate | Pseudomonas sp. | |
0.8 | - |
N-acetyl-D-galactosamine | - |
Pseudomonas sp. | |
5.3 | - |
N-acetyl-D-glucosamine | - |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | inhibitory | Pseudomonas sp. | |
additional information | not effected by Ca2+, Mg2+, Cd2+, Ni2+, Zn2+, Cu2+, Mn2+, Co2+ | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Pseudomonas sp. |
124000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-glucosamine + NAD+ | Pseudomonas sp. | - |
N-acetyl-D-glucosaminate + NADH | - |
? | |
N-acetyl-D-glucosamine + NAD+ | Pseudomonas sp. No. 53 | - |
N-acetyl-D-glucosaminate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
No. 53 | - |
Pseudomonas sp. No. 53 | - |
No. 53 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.265 | - |
crude extract | Pseudomonas sp. |
239 | - |
purified enzyme | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, several months, without much loss of activity | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-galactosamine + NAD+ | - |
Pseudomonas sp. | N-acetyl-D-galactoseaminate + NADH | - |
? | |
N-acetyl-D-galactosamine + NAD+ | - |
Pseudomonas sp. No. 53 | N-acetyl-D-galactoseaminate + NADH | - |
? | |
N-acetyl-D-glucosamine + NAD+ | - |
Pseudomonas sp. | N-acetyl-D-glucosaminate + NADH | - |
? | |
N-acetyl-D-glucosamine + NAD+ | - |
Pseudomonas sp. No. 53 | N-acetyl-D-glucosaminate + NADH | - |
? | |
N-acetyl-D-mannosamine + NAD+ + H2O | very poor substrate | Pseudomonas sp. | N-acetyl-D-mannosaminate + NADH + H+ | - |
? | |
N-acetyl-D-mannosamine + NAD+ + H2O | very poor substrate | Pseudomonas sp. No. 53 | N-acetyl-D-mannosaminate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 30000, SDS-PAGE, gel filtration | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, unstable below pH 5 and above pH 12 | Pseudomonas sp. |
55 | - |
10 min, stable | Pseudomonas sp. |
65 | - |
10 min, 50% loss of activity | Pseudomonas sp. |
70 | - |
10 min, complete loss of activity | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
655 | - |
N-acetyl-D-glucosamine | - |
Pseudomonas sp. | |
932 | - |
N-acetyl-D-galactosamine | - |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 10.5 | most stable | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strictly specific for NAD+ as hydrogen acceptor | Pseudomonas sp. |