Literature summary for 1.1.1.25 extracted from

Ye, S.; Von Delft, F.; Brooun, A.; Knuth, M.W.; Swanson, R.V.; McRee, D.E.
The crystal structure of shikimate dehydrogenase (AroE) reveals a unique NADPH binding mode (2003), J. Bacteriol., 185, 4144-4151.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and selenomethionine-labeled enzyme in Escherichia coli strain DL41	Haemophilus influenzae

Crystallization (Commentary)

Crystallization (Comment)	Organism
catalytic domain with open twisted alpha/beta motif plus NADPH binding domain with typical Rossman fold	Haemophilus influenzae
wild-type and selenomethionine-labeled enzyme, free and in complex with NADPH, sitting drop vapour diffusion method, 10 mg/m protein, with or without 5 mM NADPH, mixture in a ratio of 1:1 with crystallization solution containing 1.0 M sodium citrate, and 0.1 M 2-(cyclohexylamino)ethanesulfonic acid (CHES), pH 8.8, for the free enzyme, and 12% PEG 8000, 0.15 M calcium acetate, pH 7.8, and 0.1 M imidazole for the selenomethionine-labeled enzyme, 4°C, X-ray diffraction structure determination and analysis at 2.4 A and 1.95 A, respectively, structure modeling	Haemophilus influenzae

Crystallization (Comment)

Organism

catalytic domain with open twisted alpha/beta motif plus NADPH binding domain with typical Rossman fold

Haemophilus influenzae

wild-type and selenomethionine-labeled enzyme, free and in complex with NADPH, sitting drop vapour diffusion method, 10 mg/m protein, with or without 5 mM NADPH, mixture in a ratio of 1:1 with crystallization solution containing 1.0 M sodium citrate, and 0.1 M 2-(cyclohexylamino)ethanesulfonic acid (CHES), pH 8.8, for the free enzyme, and 12% PEG 8000, 0.15 M calcium acetate, pH 7.8, and 0.1 M imidazole for the selenomethionine-labeled enzyme, 4°C, X-ray diffraction structure determination and analysis at 2.4 A and 1.95 A, respectively, structure modeling

Haemophilus influenzae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
shikimate + NADP+	Haemophilus influenzae	-	3-dehydroshikimate + NADPH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Haemophilus influenzae	-	recombinant enzyme expressed in Escherichia coli	-
Haemophilus influenzae	P43876	gene aroE; gene aroE	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
shikimate + NADP+	-	Haemophilus influenzae	3-dehydroshikimate + NADPH + H+	-	r
shikimate + NADP+	-	Haemophilus influenzae	3-dehydroshikimate + NADPH + H+	analysis of the 3-dehydroshikimate binding site	r

Subunits

Subunits	Comment	Organism
More	three-dimensional structure analysis of AroE	Haemophilus influenzae

Synonyms

Synonyms	Comment	Organism
AroE	-	Haemophilus influenzae

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Haemophilus influenzae
NADPH	binding site determinatiion and binding mode analysis, AroE contains a Rossmann fold NADPH binding domain	Haemophilus influenzae