Cloned (Comment) | Organism |
---|---|
gene aroE, DNA and amino acid sequence determination, functional expression as soluble enzyme in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics of recombinant AroE | Mycobacterium tuberculosis | |
0.01 | - |
NADPH | pH 7.0, 25°C, recombinant AroE | Mycobacterium tuberculosis | |
0.022 | - |
NADP+ | pH 7.0, 25°C, recombinant AroE | Mycobacterium tuberculosis | |
0.031 | - |
3-dehydroshikimate | pH 7.0, 25°C, recombinant AroE | Mycobacterium tuberculosis | |
0.05 | - |
shikimate | pH 7.0, 25°C, recombinant AroE | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
1 * 27000, recombinant AroE, SDS-PAGE, 1 * 27076, recombinant AroE, mass spectrometry | Mycobacterium tuberculosis |
27076 | - |
1 * 27000, recombinant AroE, SDS-PAGE, 1 * 27076, recombinant AroE, mass spectrometry | Mycobacterium tuberculosis |
27080 | - |
recombinant AroE, mass spectrometry | Mycobacterium tuberculosis |
54150 | - |
recombinant AroE, mass spectrometry | Mycobacterium tuberculosis |
58370 | - |
recombinant AroE, gel filtration | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | A5U5Q2 | gene aroE | - |
Mycobacterium tuberculosis H37Rv | A5U5Q2 | gene aroE | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble enzyme 8.5fold from Escherichia coli strain BL21(DE3) by ion exchange and hydrophobic interaction chromatography, gel filtration, and again ion exchange chromatography, to homogeneity | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.9 | - |
purified recombinant AroE | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate + NADP+ | - |
Mycobacterium tuberculosis | 3-dehydroshikimate + NADPH + H+ | - |
? | |
shikimate + NADP+ | - |
Mycobacterium tuberculosis H37Rv | 3-dehydroshikimate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54150, recombinant AroE, mass spectrometry | Mycobacterium tuberculosis |
monomer | 1 * 27000, recombinant AroE, SDS-PAGE, 1 * 27076, recombinant AroE, mass spectrometry | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
AroE | - |
Mycobacterium tuberculosis |
SD | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
purified recombinant AroE, stable for at least 1 h | Mycobacterium tuberculosis |
55 | - |
purified recombinant AroE, 20% remaining activity after 1 h | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
NADP+ | pH 7.0, 25°C, recombinant AroE | Mycobacterium tuberculosis | |
8.2 | - |
shikimate | pH 7.0, 25°C, recombinant AroE | Mycobacterium tuberculosis | |
45 | - |
NADPH | pH 7.0, 25°C, recombinant AroE | Mycobacterium tuberculosis | |
49 | - |
3-dehydroshikimate | pH 7.0, 25°C, recombinant AroE | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mycobacterium tuberculosis |