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Literature summary for 1.1.1.250 extracted from

  • Quong, M.W.; Miyada, C.G.; Switchenko, A.C.; Goodman, T.C.
    Identification, purification, and characterization of a D-arabinitol-specific dehydrogenase from Candida tropicalis (1993), Biochem. Biophys. Res. Commun., 196, 1323-1329.
    View publication on PubMed

Application

Application Comment Organism
analysis the specificity of the enzyme makes it useful for the development of a simple and specific method for the measurement of D-arabinitol, a metabolite of the pathogenic Candida spp. which has been described as a marker for disseminated candidiasis Candida tropicalis

General Stability

General Stability Organism
Mg2+ enhances the stability Candida tropicalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
NAD+
-
Candida tropicalis
39.8
-
D-arabinitol
-
Candida tropicalis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1-5 mM, 50% increase of activity Candida tropicalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
x * 31000, SDS-PAGE Candida tropicalis

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Candida tropicalis

Reaction

Reaction Comment Organism Reaction ID
D-arabinitol + NAD+ = D-ribulose + NADH + H+ in the reverse reaction the enzyme catalyzes transfer of 4(S) hydrogen of NADH Candida tropicalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
96
-
-
Candida tropicalis

Storage Stability

Storage Stability Organism
-80°C, stable Candida tropicalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinitol + NAD+ r Candida tropicalis D-ribulose + NADH + H+
-
?
D-xylulose + NADH 1% of the activity with D-ribulose Candida tropicalis ?
-
?
Galactitol + NAD+ 1.6% of the activity with D-arabinitol Candida tropicalis ?
-
?
L-Xylulose + NADH 3% of the activity with D-ribulose Candida tropicalis ?
-
?
Xylitol + NAD+ 5% of the activity with D-arabinitol Candida tropicalis ?
-
?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Candida tropicalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10 11 oxidation of D-arabinitol Candida tropicalis

pH Range

pH Minimum pH Maximum Comment Organism
8 11 pH 8.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity, pH 10-11: maximal activity Candida tropicalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
11
-
unstable above Candida tropicalis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Candida tropicalis
NADH
-
Candida tropicalis