Application | Comment | Organism |
---|---|---|
analysis | the specificity of the enzyme makes it useful for the development of a simple and specific method for the measurement of D-arabinitol, a metabolite of the pathogenic Candida spp. which has been described as a marker for disseminated candidiasis | Candida tropicalis |
General Stability | Organism |
---|---|
Mg2+ enhances the stability | Candida tropicalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
NAD+ | - |
Candida tropicalis | |
39.8 | - |
D-arabinitol | - |
Candida tropicalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1-5 mM, 50% increase of activity | Candida tropicalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Candida tropicalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida tropicalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Candida tropicalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-arabinitol + NAD+ = D-ribulose + NADH + H+ | in the reverse reaction the enzyme catalyzes transfer of 4(S) hydrogen of NADH | Candida tropicalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
96 | - |
- |
Candida tropicalis |
Storage Stability | Organism |
---|---|
-80°C, stable | Candida tropicalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinitol + NAD+ | r | Candida tropicalis | D-ribulose + NADH + H+ | - |
? | |
D-xylulose + NADH | 1% of the activity with D-ribulose | Candida tropicalis | ? | - |
? | |
Galactitol + NAD+ | 1.6% of the activity with D-arabinitol | Candida tropicalis | ? | - |
? | |
L-Xylulose + NADH | 3% of the activity with D-ribulose | Candida tropicalis | ? | - |
? | |
Xylitol + NAD+ | 5% of the activity with D-arabinitol | Candida tropicalis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Candida tropicalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | 11 | oxidation of D-arabinitol | Candida tropicalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | pH 8.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity, pH 10-11: maximal activity | Candida tropicalis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
11 | - |
unstable above | Candida tropicalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Candida tropicalis | |
NADH | - |
Candida tropicalis |