Literature summary for 1.1.1.255 extracted from

Stoop, J.M.H.; Williamson, J.D.; Conkling, M.A.; MacKay, J.J.; Pharr, D.M.
Characterization of NAD-dependent mannitol dehydrogenase from celery as affected by ions, chelators, reducing agents and metabolites (1998), Plant Sci., 131, 43-51.

No PubMed abstract available

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Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	dose dependent decrease in enzyme activity	Apium graveolens
1,4-dithioerythritol	1.09 mM, 50% inhibition, partially reversed by diamide	Apium graveolens
1,4-dithiothreitol	0.57 mM, 50% inhibition, reversed by diamide	Apium graveolens
2-mercaptoethanol	30 mM, 50% inhibition, partially reversed by diamide	Apium graveolens
ADP	Ki: 0.24 mM at pH 7.0	Apium graveolens
AMP	Ki: 0.50-0.80 mM at pH 9.5, Ki = 0.64 mM at pH 7.0	Apium graveolens
ATP	Ki: 1.10 mM at pH 7.0	Apium graveolens
additional information	EDTA and EGTA did not affect enzyme activity	Apium graveolens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00127	-	NADH	-	Apium graveolens
0.14	-	NAD+	at pH 9.5	Apium graveolens
0.23	-	NAD+	at pH 7.0	Apium graveolens
64	-	D-mannitol	at pH 9.5	Apium graveolens
400	-	D-mannitol	at pH 7.0	Apium graveolens
745	-	D-mannose	-	Apium graveolens

Organism

Organism	UniProt	Comment	Textmining
Apium graveolens	-	L. var. dulce Mill. Pers.	-
Apium graveolens	-	celery	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.9	-	at pH 9.5	Apium graveolens
2.2	-	at pH 7.0	Apium graveolens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-mannitol + NAD+	-	Apium graveolens	D-mannose + NADH + H+	-	r
additional information	no cinnamyl alcohol dehydrogenase activity	Apium graveolens	?	-	?

Synonyms

Synonyms	Comment	Organism
MTD	-	Apium graveolens

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	-	D-mannose reduction	Apium graveolens
9.5	-	D-mannitol oxidation	Apium graveolens

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Apium graveolens

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Release 2023.1 (January 2023)