Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a E141N/Q313E mutant formate dehydrogenase which gains glyoxylate reductase activity improved through enhancement of the hydrogen transfer step in the catalytic process, while the replacement induces no marked activity toward other 2-koxoacid substrates, and diminishes the enzyme's activity as to formate oxidation, substrate specificity and kinetic switch of the mutant, overview | Paracoccus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.4 | - |
glyoxylate | pH 7.0, 30°C, recombinant E141N/Q313E mutant formate dehydrogenase | Paracoccus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NADH | a E141N/Q313E mutant formate dehydrogenase shows glyoxylate reductase activity improved through enhancement of the hydrogen transfer step in the catalytic process, overview | Paracoccus sp. | glycolate + NAD+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at, E141N/Q313E mutant formate dehydrogenase | Paracoccus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
glyoxylate | pH 7.0, 30°C, recombinant E141N/Q313E mutant formate dehydrogenase | Paracoccus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at, E141N/Q313E mutant formate dehydrogenase | Paracoccus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Paracoccus sp. |