Crystallization (Comment) | Organism |
---|---|
55% ammonium sulfate precipitation | Gluconobacter oxydans |
General Stability | Organism |
---|---|
highly unstable, 75% loss of activity within a few days, stabilizing agents during purification: D-gluconate, 5-keto-D-gluconate | Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Gluconobacter oxydans | |
Cs2+ | - |
Gluconobacter oxydans | |
Cu2+ | - |
Gluconobacter oxydans | |
Hg2+ | - |
Gluconobacter oxydans | |
Ni2+ | - |
Gluconobacter oxydans | |
p-chloromercuribenzoate | - |
Gluconobacter oxydans | |
Sn2+ | - |
Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NADPH | - |
Gluconobacter oxydans | |
0.02 | - |
NADP+ | - |
Gluconobacter oxydans | |
0.9 | - |
5-Keto-D-gluconate | - |
Gluconobacter oxydans | |
20 | - |
D-gluconate | - |
Gluconobacter oxydans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | slight activation at 1 mM | Gluconobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
4 * 25000, SDS-PAGE | Gluconobacter oxydans |
110000 | - |
gel filtration | Gluconobacter oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-idonate + NADP+ | Gluconobacter oxydans | - |
5-dehydro-D-gluconate + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, crystalline, chromatography techniques | Gluconobacter oxydans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Gluconobacter oxydans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
69.7 | - |
- |
Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-keto-D-fructose + NADP+ | low activity | Gluconobacter oxydans | ? + NADPH | - |
? | |
D-gluconate + NADP+ | - |
Gluconobacter oxydans | ? + NADPH | - |
r | |
L-idonate + NADP+ | - |
Gluconobacter oxydans | 5-dehydro-D-gluconate + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 25000, SDS-PAGE | Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
5-keto-D-gluconate reduction | Gluconobacter oxydans |
10 | - |
D-gluconate oxidation | Gluconobacter oxydans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | 80% of maximal activity within | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Gluconobacter oxydans |