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Literature summary for 1.1.1.27 extracted from

  • Prachayasittikul, V.; Ljung, S.; Isarankura-Na-Ayudhya, C.; Buelow, L.
    NAD(H) recycling activity of an engineered bifunctional enzyme galactose dehydrogenase/lactate dehydrogenase (2006), Int. J. Biol. Sci., 2, 10-16.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology a chimeric bifunctional enzyme composing of galactose dehydrogenase from Pseudomonas fluorescens and lactate dehydrogenase from Bacillus stearothermophilus is successfully constructed. The chimeric enzyme is able to recycle NAD with a continuous production of lactate without any externally added NADH Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
a chimeric bifunctional enzyme composing of galactose dehydrogenase from Pseudomonas fluorescens and lactate dehydrogenase from Bacillus stearothermophilus is successfully constructed and expressed in Escherichia coli Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
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